Evidence map›Paper›PMID 35845313›Full record

ArticleSynthetic and systems biotechnology2022

Sequence and thermodynamic characteristics of terminators revealed by FlowSeq and the discrimination of terminators strength.

Weiji Zhai, Yanting Duan, Xiaomei Zhang, Guoqiang Xu, Hui Li, Jinsong Shi, Zhenghong Xu, Xiaojuan Zhang

Open access · goldAbstract read
In one paragraph

Article in Synthetic and systems biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.9field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 18 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Weiji ZhaiBiotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
Yanting DuanBiotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
Xiaomei ZhangSchool of Life Science and Health Engineering, Jiangnan University, Wuxi, 214122, China.
Guoqiang XuBiotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
Hui LiSchool of Life Science and Health Engineering, Jiangnan University, Wuxi, 214122, China.
Jinsong ShiSchool of Life Science and Health Engineering, Jiangnan University, Wuxi, 214122, China.
Zhenghong XuBiotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
Xiaojuan ZhangBiotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
Jiangnan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The intrinsic terminator in prokaryotic forms secondary RNA structure and terminates the transcription. However, leaking transcription is common due to varied terminator strength. Besides of the representative hairpin and U-tract structure, detailed sequence and thermodynamic features of terminators were not completely clear, and the effect of terminator on the upstream gene expression was unclearly. Thus, it is still challenging to use terminator to control expression with higher precision. Here, in

Indexed as

FlowSeqFree energyIntrinsic terminatorMachine learningStructure-activity relationshipTranscription termination

Identifiers

PMID35845313
PMCPMC9257418
OpenAlexW4283211993

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.