Evidence map›Paper›PMID 35844419›Full record

ArticleSaudi journal of biological sciences2022

Hypoxia, a dynamic tool to amplify the gingival mesenchymal stem cells potential for neurotrophic factor secretion.

Shankargouda Patil, Hytham N Fageeh, Hammam Ibrahim Fageeh, Wael Ibraheem, Abdulrahman Saleh Alshehri, Ashraf Al-Brakati, Salem Almoammar, Mohammad Almagbol, Harisha Dewan, Samar Saeed Khan and 4 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in Saudi journal of biological sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 1 pooled it
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 1 synthesis or guideline pooled it, 11 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review
  4. Article
  5. Mesenchymal stem cells and their microenvironment.Stem cell research & therapy · 2022
    Review
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 5 institutions in 2 countries.

Shankargouda PatilDepartment of Maxillofacial Surgery and Diagnostic Sciences, Division of Oral Pathology, College of Dentistry, Jazan University, Jazan, Saudi Arabia.
Hytham N FageehDepartment of Preventive Dental Sciences, College of Dentistry, Jazan University, Jazan, Saudi Arabia.
Hammam Ibrahim FageehDepartment of Preventive Dental Sciences, College of Dentistry, Jazan University, Jazan, Saudi Arabia.
Wael IbraheemDepartment of Preventive Dental Sciences, College of Dentistry, Jazan University, Jazan, Saudi Arabia.
Abdulrahman Saleh AlshehriDepartment of Preventive Dental Sciences, College of Dentistry, Jazan University, Jazan, Saudi Arabia.
Ashraf Al-BrakatiDepartment of Human Anatomy, College of Medicine, Taif University, Taif, Saudi Arabia.
Salem AlmoammarDepartment of Pediatric Dentistry and Orthodontics, College of Dentistry, King Khalid University, Abha, Saudi Arabia.
Mohammad AlmagbolDepartment of Communirty and Eriodontics, Faculty of Dentistry, King Khalid University, Abha, Saudi Arabia.
Harisha DewanDepartment of Prosthetic Dental Sciences, College of Dentistry, Jazan University, Jazan, Saudi Arabia.
Samar Saeed KhanDepartment of Maxillofacial Surgery and Diagnostic Sciences, Division of Oral Pathology, College of Dentistry Jazan University, Jazan, Saudi Arabia.
Hosam Ali BaeshenDepartment of Orthodontics, College of Dentistry, King Abdulaziz University, Jeddah, Saudi Arabia.
Vikrant R PatilBiogenre Private Limited, Pune, India.
A Thirumal RajDepartment of Oral Pathology and Microbiology, Sri Venkateswara Dental College and Hospital, Chennai, India.
Shilpa BhandiDepartment of Restorative Dental Science, Division of Operative Dentistry, College of Dentistry, Jazan University, Jazan, Saudi Arabia.
Jazan University · SAKing Khalid University · SAKing Abdulaziz University · SASri Venkateswara Medical College and Ruia Hospital · INTaif University · SA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Gingival mesenchymal stem cells (GMSCs) have significant regenerative potential. Their potential applications range from the treatment of inflammatory diseases, wound healing, and oral disorders. Preconditioning these stem cells can optimize their biological properties. Hypoxia preconditioning of MSCs improves stem cell properties like proliferation, survival, and differentiation potential. This research explored the possible impact of hypoxia on the pluripotent stem cell properties that GMSCs possess. We evaluated the morphology, stemness, neurotrophic factors, and stemness-related genes. We compared the protein levels of secreted neurotrophic factors between normoxic and hypoxic GMSC-conditioned media (GMSC-CM). Results revealed that hypoxic cultured GMSC's had augmented expression of neurotrophic factors BDNF, GDNF, VEGF, and IGF1 and stemness-related gene NANOG. Hypoxic GMSCs showed decreased expression of the OCT4 gene. In hypoxic GMSC-CM, the neurotrophic factors secretions were significantly higher than normoxic GMSC-CM. Our data demonstrate that culturing of GMSCs in hypoxia enhances the secretion of neurotrophic factors that can lead to neuronal lineage differentiation.

Indexed as

Gingival mesenchymal stem cellHypoxiaNeurotrophic factorsNormoxia

Identifiers

PMID35844419
PMCPMC9280216
OpenAlexW4214654142

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.