ArticleMetabolomics : Official journal of the Metabolomic Society2022
Non-Invasive Identification of Sex in Cultured Bovine Embryos by UHPLC-MS/MS Metabolomics.
Article in Metabolomics : Official journal of the Metabolomic Society, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 8 citations in OpenAlex.
- Metabolomic Markers Reveal How hCG-Ketoprofen Intervention Increase Pregnancy Percentage Following Timed Artificial Insemination in Dairy Cows.Animals : an open access journal from MDPI · 2026Article
- In vitro production significantly reduces metabolic differences among bovine embryos.Metabolomics : Official journal of the Metabolomic Society · 2025Article
- Progress of sex control techniques in mammals†.Biology of reproduction · 2025Review
- The recipient metabolome explains the asymmetric ovarian impact on fetal sex development after embryo transfer in cattle.Journal of animal science · 2024Article
- Metabolomic profiling implicates mitochondrial and immune dysfunction in disease syndromes of the critically endangered black rhinoceros (Diceros bicornis).Scientific reports · 2023Article
- The Metabolic Signature of In Vitro Produced Bovine Embryos Helps Predict Pregnancy and Birth after Embryo Transfer.Metabolites · 2021Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionDifferent gene expression between male and female bovine embryos leads to metabolic differences.
objectiveWe used UHPLC-MS/MS to identify sex metabolite biomarkers in embryo culture medium (CM).
methodsEmbryos were produced in vitro under highly variable conditions, i.e., fertilized with 7 bulls, two breeds, and cultured with BSA or BSA + serum until Day-6. On Day-6, embryos were cultured individually for 24 h. CM of Day-7 embryos (86 female and 81 male) was collected, and Day-6 and Day-7 embryonic stages recorded.
resultsA study by sample subsets with fixed factors (culture, bull breed, and Day-6 and Day-7 stages) tentatively identified 31 differentially accumulated metabolites through 182 subsets. Day-6 and Day-7 stage together affected 13 and 11 metabolites respectively, while 19 metabolites were affected by one or another stage and/or day. Culture supplements and individual bull changed 19 and 15 metabolites, respectively. Single bull exerted the highest influence (20 metabolites with the significantly highest p values). Lipid (93 subsets; 11 metabolites) and amino acid (55 subsets; 13 metabolites) were the most relevant classes for sex identification.
conclusionsSingle biomarker led to inefficient sex diagnosis, while metabolite combinations accurately identified sex. Our study is a first in non-invasive sex identification in cattle by overcoming factors that induce metabolic variation.
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