ArticleThe Journal of cell biology1987
The platelet fibrinogen receptor: an immunogold-surface replica study of agonist-induced ligand binding and receptor clustering.
Article in The Journal of cell biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 184 citations in OpenAlex.
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- The anticoagulant properties of cadmium telluride quantum dots.Journal of interdisciplinary nanomedicine · 2018Article
- RGDfK-functionalized gold nanorods bind only to activated platelets.Journal of biomedical materials research. Part A · 2017Article
- Current concepts of platelet activation: possibilities for therapeutic modulation of heterotypic vs. homotypic aggregation.British journal of clinical pharmacology · 2011Review
- A few immobilized thrombins are sufficient for platelet spreading.Biophysical journal · 2011Article
- Two-color nanoscopy of three-dimensional volumes by 4Pi detection of stochastically switched fluorophores.Nature methods · 2011Article
- Integrin alphaIIbbeta3 activation in Chinese hamster ovary cells and platelets increases clustering rather than affinity.The Journal of biological chemistry · 2010Article
- Disrupting integrin transmembrane domain heterodimerization increases ligand binding affinity, not valency or clustering.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- The primacy of affinity over clustering in regulation of adhesiveness of the integrin {alpha}L{beta}2.The Journal of cell biology · 2004Article
- Complementary roles for receptor clustering and conformational change in the adhesive and signaling functions of integrin alphaIIb beta3.The Journal of cell biology · 1998Article
- Defining extracellular integrin alpha-chain sites that affect cell adhesion and adhesion strengthening without altering soluble ligand binding.Molecular biology of the cell · 1997Article
- Expression of alpha v and beta 3 integrin chains on murine lymphocytes.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- The integrin alpha IIb beta 3 contains distinct and interacting binding sites for snake-venom RGD (Arg-Gly-Asp) proteins. Evidence that the receptor-binding characteristics of snake-venom RGD proteins are related to the amino acid environment flanking the sequence RGD.The Biochemical journal · 1995Article
- Bound fibrinogen distribution on stimulated platelets. Examination by confocal scanning laser microscopy.The American journal of pathology · 1995Article
- Absence of ligands bound to glycoprotein IIB-IIIA on the exposed surface of a thrombus may limit thrombus growth in flowing blood.The Journal of clinical investigation · 1994Article
- Glycoprotein IIb-IIIa and the translocation of Rap2B to the platelet cytoskeleton.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Proteolytic degradation of the RGD-binding and non-RGD-binding conformers of human platelet integrin glycoprotein IIb/IIIa: clues for identification of regions involved in the receptor's activation.The Biochemical journal · 1994Article
- Studies of activated GPIIb/IIIa receptors on the luminal surface of adherent platelets. Paradoxical loss of luminal receptors when platelets adhere to high density fibrinogen.The Journal of clinical investigation · 1993Article
- Evidence for the selective association of a subpopulation of GPIIb-IIIa with the actin cytoskeletons of thrombin-activated platelets.The Journal of cell biology · 1993Article
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Authors and funding
5 authors.
Funding
Abstract
Platelet aggregation requires the binding of fibrinogen to its receptor, a heterodimer consisting of the plasma-membrane glycoproteins (GP) IIb and IIIa. Although the GPIIb-IIIa complex is present on the surface of unstimulated platelets, it binds fibrinogen only after platelet activation. We have used an immunogold-surface replica technique to study the distribution of GPIIb-IIIa and bound fibrinogen over broad areas of surface membranes in unstimulated, as well as thrombin-activated and ADP-activated human platelets. We found that the immunogold-labeled GPIIb-IIIa was monodispersed over the surface of unstimulated platelets, although the cell surface lacked immunoreactive fibrinogen. On thrombin-stimulated platelets, approximately 65% of the GPIIb-IIIa molecules were in clusters within the plane of the membrane. Fibrinogen, which had been released from the alpha-granules of these cells, bound to GPIIb-IIIa on the cell surface and was similarly clustered. To determine whether the receptors clustered before ligand binding, or as a consequence thereof, we studied the surface distribution of GPIIb-IIIa after stimulation with ADP, which causes activation of the fibrinogen receptor function of GPIIb-IIIa without inducing the release of fibrinogen. In the absence of added fibrinogen, the unoccupied, yet binding-competent receptors on ADP-stimulated platelets were monodispersed. The addition of fibrinogen caused the GPIIb-IIIa molecules to cluster on the cell surface. Clustering was also induced by the addition of the GPIIb-IIIa-binding domains of fibrinogen, namely the tetrapeptide Arg-Gly-Asp-Ser on the alpha-chain or the gamma-chain decapeptide gamma 402-411. These results show that receptor occupancy causes clustering of GPIIb-IIIa in activated platelets.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.