Evidence map›Paper›PMID 35841132›Full record

ArticleJournal of medical primatology2022

Optimization and use of near infrared imaging to guide lymph node collection in rhesus macaques (Macaca mulatta).

Jeremy V Smedley, Rachele M Bochart, Miranda Fischer, Heidi Funderburgh, Vanessa Kelly, Hugh Crank, Kim Armantrout, Oriene Shiel, Mitchell Robertson-LeVay, Nikki Sternberger and 20 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of medical primatology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

30 authors at 3 institutions in 1 country.

Jeremy V SmedleyInfectious Disease Resource, Division of Pathobiology and Immunology, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.ORCID 0000-0003-3369-4662
Rachele M BochartInfectious Disease Resource, Division of Pathobiology and Immunology, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
Miranda FischerInfectious Disease Resource, Division of Pathobiology and Immunology, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
Heidi FunderburghInfectious Disease Resource, Division of Pathobiology and Immunology, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
Vanessa KellyInfectious Disease Resource, Division of Pathobiology and Immunology, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
Hugh CrankInfectious Disease Resource, Division of Pathobiology and Immunology, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
Kim ArmantroutInfectious Disease Resource, Division of Pathobiology and Immunology, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
Oriene ShielInfectious Disease Resource, Division of Pathobiology and Immunology, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
Mitchell Robertson-LeVaySurgical Services Unit, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
Nikki SternbergerSurgical Services Unit, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
Brian SchmalingSurgical Services Unit, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
Sheila RobertsSurgical Services Unit, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
Vicki SekiguchiSurgical Services Unit, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
Michael ReuszSurgical Services Unit, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
Tiah SchwartzSurgical Services Unit, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
Kimberly A MeyerCenter for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA.
Gabriela WebbVaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USA.
Roxanne M GilbrideVaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USA.
Nicholas DambrauskasCenter for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA.
Daniela AndradeCenter for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA.
Matthew WoodCenter for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA.
Caralyn LabriolaExperimental Pathology Unit, Division of Pathobiology and Immunology, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
Michael AxthelmExperimental Pathology Unit, Division of Pathobiology and Immunology, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
Nina DerbyCenter for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA.
Ben Varco-MerthVaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USA.
Yoshinori FukazawaVaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USA.
Scott HansenVaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USA.
Jonah B SachaVaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USA.
Donald L SodoraCenter for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA.
D Noah SatherCenter for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA.
Oregon National Primate Research Center · USInfectious Disease Research Institute · USOregon Health & Science University · US

Funding

Upgrade of confocal microscopy at the Oregon National Primate Research CenterP51OD011092 · OD · OREGON HEALTH & SCIENCE UNIVERSITY · PI Bonnie J. Nagel · 2012 to 2026
$203.9M
Phase 2 Enhancement of Outdoor Group Housing for U42 SPF 9 Rhesus MacaquesU42OD023038 · OD · OREGON HEALTH & SCIENCE UNIVERSITY · PI RUDOLF Paul BOHM, Lauren Drew Martin · 2016 to 2026
$21.9M
Diseases of Public Health Importance Training GrantT32AI007509 · NIAID · UNIVERSITY OF WASHINGTON · PI LUND, JENNIFER M · 1997 to 2024
$6.3M
Viral Testing CoreU42OD010426 · OD · OREGON HEALTH & SCIENCE UNIVERSITY · PI Andrew Haertel, Lauren Drew Martin · 2013 to 2026
$6.0M
Harnessing oral mucosa vaccination to drive protective HIV antibody responsesR01DE026336 · NIDCR · SEATTLE CHILDREN'S HOSPITAL · PI SATHER, D. NOAH, SODORA, DONALD L · 2016 to 2020
$4.1M
NIAID NIH HHS T32 AI007509NIDCR NIH HHS R01 DE026336NIH HHS P51 OD011092NIH HHS U42 OD010426NIH HHS U42 OD023038
6 · The paper itself

Abstract

backgroundIdentification of lymph nodes (LNs) draining a specific site or in obese macaques can be challenging.

methodsIndocyanine Green (ICG) was administered intradermal (ID), intramuscular, in the oral mucosa, or subserosal in the colon followed by Near Infrared (NIR) imaging.

resultsAfter optimization to maximize LN identification, intradermal ICG was successful in identifying 50-100% of the axillary/inguinal LN at a site. Using NIR, collection of peripheral and mesenteric LNs in obese macaques was 100% successful after traditional methods failed. Additionally, guided collection of LNs draining the site of intraepithelial or intramuscular immunization demonstrated significantly increased numbers of T follicular helper (Tfh) cells in germinal centers of draining compared to nondraining LNs.

conclusionThese imaging techniques optimize our ability to evaluate immune changes within LNs over time, even in obese macaques. This approach allows for targeted serial biopsies that permit confidence that draining LNs are being harvested throughout the study.

Indexed as

Indocyanine GreenLymph NodesAnimalsMacaca mulattaObesityIndocyanine GreendrainingfluorescenceICGimagingindocyanine greenlymph nodemacaquemesentericMLNtargeted sampling

Identifiers

PMID35841132
PMCPMC9474636
OpenAlexW4285589253

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.