ArticleMolecular ecology resources2023
A consensus protocol for the recovery of mercury methylation genes from metagenomes.
Article in Molecular ecology resources, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 33 citations in OpenAlex.
- Metagenomics reveals diverse community of putative mercury methylators across different biogeochemical niches in Sansha Yongle blue hole.Marine life science & technology · 2026Article
- Genomic potential for mercury biotransformation in marine sediments across marginal slope to hadal zone.Nature communications · 2025Article
- The role of prokaryotic mercury methylators and demethylators in Canadian Arctic thermokarst lakes.Scientific reports · 2025Article
- S-adenosyl-L-methionine is the unexpected methyl donor for the methylation of mercury by the membrane-associated HgcAB complex.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Recent advance of microbial mercury methylation in the environment.Applied microbiology and biotechnology · 2024Review
- Phylogenetic and ecophysiological novelty of subsurface mercury methylators in mangrove sediments.The ISME journal · 2023Article
- Redox gradient shapes the abundance and diversity of mercury-methylating microorganisms along the water column of the Black Sea.mSystems · 2023Article
- Transcriptional Control ofApplied and environmental microbiology · 2023Article
- On the Origin and Evolution of Microbial Mercury Methylation.Genome biology and evolution · 2023Article
- Potential for mercury methylation by Asgard archaea in mangrove sediments.The ISME journal · 2023Article
- A consensus protocol for the recovery of mercury methylation genes from metagenomes.Molecular ecology resources · 2023Article
Corrections and comments
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Authors and funding
29 authors at 18 institutions in 9 countries.
Funding
Abstract
Mercury (Hg) methylation genes (hgcAB) mediate the formation of the toxic methylmercury and have been identified from diverse environments, including freshwater and marine ecosystems, Arctic permafrost, forest and paddy soils, coal-ash amended sediments, chlor-alkali plants discharges and geothermal springs. Here we present the first attempt at a standardized protocol for the detection, identification and quantification of hgc genes from metagenomes. Our Hg-cycling microorganisms in aquatic and terrestrial ecosystems (Hg-MATE) database, a catalogue of hgc genes, provides the most accurate information to date on the taxonomic identity and functional/metabolic attributes of microorganisms responsible for Hg methylation in the environment. Furthermore, we introduce "marky-coco", a ready-to-use bioinformatic pipeline based on de novo single-metagenome assembly, for easy and accurate characterization of hgc genes from environmental samples. We compared the recovery of hgc genes from environmental metagenomes using the marky-coco pipeline with an approach based on coassembly of multiple metagenomes. Our data show similar efficiency in both approaches for most environments except those with high diversity (i.e., paddy soils) for which a coassembly approach was preferred. Finally, we discuss the definition of true hgc genes and methods to normalize hgc gene counts from metagenomes.
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