ArticlePloS one2022
Rapid and zero-cost DNA extraction from soft-bodied insects for routine PCR-based applications.
Article in PloS one, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 26 citations in OpenAlex.
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- Development and pilot application of a point-of-need molecular xenomonitoring protocol for tsetse (Glossina sp.) in a low-resource setting.PLoS neglected tropical diseases · 2026Article
- Simple DNA extraction for museum beetle specimens to unlock genetic data from historical collections.MethodsX · 2025Article
- A non-destructive, fast, inexpensive, non-toxic chelating resin-based DNA extraction protocol for insect voucher specimens and associated microbiomes.Journal of insect science (Online) · 2025Article
- One-Step Multiplex Polymerase Chain Reaction Assay for the Detection of Major Disease-Transmitting Mosquito Vectors in India.The American journal of tropical medicine and hygiene · 2025Article
- Co-occurrence of native and invasive malaria vectors in anthropogenic habitats in Metehara, Ethiopia: Opportunities for urban malaria control.Current research in parasitology & vector-borne diseases · 2025Article
- Evaluation of Protocols for DNA Extraction from IndividualMethods and protocols · 2024Article
- Rapid and Accurate Detection ofInsects · 2024Article
- Streamlined boiling lysis DNA extraction for Gram-positive aquaculture pathogen Streptococcus agalactiae.Archives of microbiology · 2024Article
- Unearthing Lactococcus lactis and Scheffersomyeces symbionts from edible wood-boring beetle larvae as a bio-resource for industrial applications.BMC microbiology · 2024Article
- FlashPCR: Revolutionising qPCR by Accelerating Amplification through Low ∆T Protocols.International journal of molecular sciences · 2024Article
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- Effect of silencingFrontiers in microbiology · 2023Article
- Genomic Characterization of Endosymbiotic Bacteria Associated WithBioinformatics and biology insights · 2023Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleic acid extraction is the first and foremost step in molecular biology studies. Extraction of DNA from small, soft-bodied insects is often time-consuming and costly. A fast, easy, and cost-effective DNA extraction method with greater yield and purity of DNA would aid in the rapid diagnostics, screening of large populations, and other routine PCR-based applications. The present study evaluated and standardized a rapid and zero-cost DNA extraction from soft-bodied small insects for routine molecular studies. Five rapid DNA extraction methods viz. extraction in sterile distilled water (SDW), 1X phosphate-buffered saline (PBS, pH 7.4), 1.4 M sodium chloride (NaCl), 20 mM ethylenediaminetetraacetic acid (EDTA, pH 8.0), and elution from blotted nitrocellulose membrane (NCM) were compared with standard CTAB extraction buffer and DNeasy® Blood and Tissue Kit methods. The average yield, purity, storage stability, time, and cost of extraction were assessed for all the methods and compared. A method of DNA extraction by simply crushing the soft-bodied insects in SDW was ideal in terms of yield, purity, storability, and performing routine PCR-based applications including detection of pathogens from vector species. The extraction could be accomplished in 2.5 min only with zero-reagent cost. The method would be useful in rapid molecular diagnostics and screening large populations of soft-bodied insects.
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