Evidence map›Paper›PMID 35836766›Full record

ArticleJournal of clinical and translational hepatology2022

Hepatitis B Virus Induces Microtubule Stabilization to Promote Productive Infection through Upregulating Microtubule-associated Protein 1S.

Yuanyue Guan, Bin Sun, Shijie Zhang, Yuan Zhuang, Yanxiang Huang, Minghua Lin, Rongling Zheng, Dexi Chen, Ying Shi, Yanjun Wang

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical and translational hepatology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. Unraveling the Dynamic Role of Microtubules in the HBV Life Cycle.Journal of clinical and translational hepatology · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Yuanyue GuanDepartment of Beijing Institute of Hepatology, Beijing You An Hospital, Capital Medical University, Beijing, China.
Bin SunDepartment of Intervention Therapy Center of Tumor and Liver Diseases, Beijing You An Hospital, Capital Medical University, Beijing, China.
Shijie ZhangDepartment of Pathology, Beijing You An Hospital, Capital Medical University, Beijing, China.
Yuan ZhuangDepartment of Beijing Institute of Hepatology, Beijing You An Hospital, Capital Medical University, Beijing, China.
Yanxiang HuangClinical Laboratory Center, Beijing You An Hospital, Capital Medical University, Beijing, China.
Minghua LinDepartment of Beijing Institute of Hepatology, Beijing You An Hospital, Capital Medical University, Beijing, China.
Rongling ZhengDepartment of Beijing Institute of Hepatology, Beijing You An Hospital, Capital Medical University, Beijing, China.
Dexi ChenDepartment of Beijing Institute of Hepatology, Beijing You An Hospital, Capital Medical University, Beijing, China.
Ying ShiDepartment of Beijing Institute of Hepatology, Beijing You An Hospital, Capital Medical University, Beijing, China.ORCID https://orcid.org/0000-0001-5838-0817
Yanjun WangDepartment of Beijing Institute of Hepatology, Beijing You An Hospital, Capital Medical University, Beijing, China.ORCID https://orcid.org/0000-0002-9602-6816
Capital University · USBeijing YouAn Hospital · CNCapital Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background and Aims: Continuous release and transmission of hepatitis B virus (HBV) is one of the main factors leading to chronic hepatitis B (CHB) infection. However, the mechanism of HBV-host interaction for optimal viral transport is unclear. Hence, we aimed to explore how HBV manipulates microtubule-associated protein 1S (MAP1S) and microtubule (MT) to facilitate its transport and release. Methods: The expression of MAP1S or acetylated MT was investigated by immunofluorescence, RT-PCR, immunoblotting, and plasmid transfection. MAP1S overexpression or knockdown was performed by lentiviral infection or sh-RNA transfection, respectively. HBV DNA was quantified using q-PCR. Results: Significantly higher level of MAP1S in HepG2215 cells compared with HepG2 cells was detected using RT-PCR ( Conclusions: These results demonstrate that HBx induces the formation of stable MTs to promote the release of HBV particles through upregulating MAP1S. Thus, our studies delineate a unique molecular pathway through which HBV manipulates the cytoskeleton to facilitate its own transportation, and indicate the possibility of targeting MAP1S pathway for treatment of patients with CHB.

Indexed as

Hepatitis B virusHepatitis B virus X proteinMicrotubuleMicrotubule-associated protein 1S

Identifiers

PMID35836766
PMCPMC9240238
OpenAlexW3174997972

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.