ArticleBMC plant biology2022
Chromosome-scale genome assembly of Rhododendron molle provides insights into its evolution and terpenoid biosynthesis.
Article in BMC plant biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed.
- Pangenome analysis reveals the genetic mechanism underlying high-altitude adaptation in Qinghai-Xizang (Tibet) Plateau Rhododendron.Journal of integrative plant biology · 2026Article
- Distinct evolutionary trajectories of gene duplication modes in 40 diploid cotton genomes.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Chromosome-level genome assembly and annotation of the endangered plant Primula kwangtungensis (Primulaceae).Scientific data · 2026Article
- Adaptive Traits and Molecular Mechanisms ofPlants (Basel, Switzerland) · 2025Article
- OfWRKY33 binds to the promoter of key linalool synthase geneHorticulture research · 2025Article
- Integration of metabolomics and transcriptomics unravels the identification ofFrontiers in plant science · 2025Article
- Combined metabolomic and transcriptomic analysis reveals the key genes for triterpenoid biosynthesis in Cyclocarya paliurus.BMC genomics · 2024Article
- A haplotype-resolved genome assembly of Rhododendron vialii based on PacBio HiFi reads and Hi-C data.Scientific data · 2023Article
- Gapless genome assembly of azalea and multi-omics investigation into divergence between two species with distinct flower color.Horticulture research · 2023Article
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10 authors.
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Abstract
backgroundRhododendron molle (Ericaceae) is a traditional Chinese medicine, which has been used to treat rheumatism and relieve pain since ancient times. The characteristic grayanoids of this plant have been demonstrated to be the chemical basis for the analgesic activity. Moreover, unlike morphine, these diterpenoids are non-addictive. Grayanoids mainly distribute in the leaves, flowers, roots, and fruits of R. molle, with low content. Currently the research on the biosynthesis of grayanoids is hindered, partially due to lack of the genomic information.
resultsIn the present study, a total of 744 Mb sequences were generated and assembled into 13 chromosomes. An ancient whole-genome duplication event (Ad-β) was discovered that occurred around 70 million years ago. Tandem and segmental gene duplications led to specific gene expansions in the terpene synthase and cytochrome P450 (CYP450) gene families. Two diterpene synthases were demonstrated to be responsible for the biosynthesis of 16α-hydroxy-ent-kaurane, the key precursor for grayanoids. Phylogenetic analysis revealed a species-specific bloom of the CYP71AU subfamily, which may involve the candidate CYP450s responsible for the biosynthesis of grayanoids. Additionally, three putative terpene biosynthetic gene clusters were found.
conclusionsWe reported the first genome assembly of R. molle and investigated the molecular basis underpinning terpenoids biosynthesis. Our work provides a foundation for elucidating the complete biosynthetic pathway of grayanoids and studying the terpenoids diversity in R. molle.
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