Evidence map›Paper›PMID 35836084›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Compact Eight-Channel Light-Sensing System for Bioassays.

Sung-Bae Kim, Sharon Seiko Hori, Negar Sadeghipour, Uday Kumar Sukumar, Ramasamy Paulmurugan

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Sung-Bae KimEnvironmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan. kimu-sb@aist.go.jp.
Sharon Seiko HoriMolecular Imaging Program at Stanford, Stanford University School of Medicine, Palo Alto, CA, USA.
Negar SadeghipourMolecular Imaging Program at Stanford, Stanford University School of Medicine, Palo Alto, CA, USA.
Uday Kumar SukumarMolecular Imaging Program at Stanford, Stanford University School of Medicine, Palo Alto, CA, USA.
Ramasamy PaulmuruganMolecular Imaging Program at Stanford, Stanford University School of Medicine, Palo Alto, CA, USA. paulmur8@stanford.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present protocol introduces a new instrumental setup as a luminometer to simultaneously measure eight light samples with high sensitivity. The system consists of 8-channel photomultiplier tubes (8-PMTs) with different sensitivities to light. Therefore, it is critical to normalize the sensitivities of PMTs to light samples and integrate them as a system. We first introduce how to normalize the diverse light sensitivity among the PMTs using placental alkaline phosphatase (PLAP) as a model chemiluminescence light source. The normalized BBI system shows a statistically strong linear correlation graph to photon counts. The biomedical utility of this system is exemplified by (i) determining the alkaline phosphatase (AP) activities in mouse plasma samples as a cancer biomarker and (ii) diagnosing metastatic tissues during cancer progression using bioluminescent reporter.

Indexed as

Alkaline PhosphatasePlacentaAnimalsBiological AssayBiomarkers, TumorFemaleMicePhotonsPregnancyAlkaline PhosphataseBiomarkers, TumorBlack Box I (BBI)ChemiluminescenceEight-channelLuminescenceOn-site analysisPhotomultiplier tubes (PMTs)

Identifiers

PMID35836084

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.