Evidence map›Paper›PMID 35836077›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

In Vivo Imaging of Oxidative and Hypoxic Stresses in Mice Model of Amyotrophic Lateral Sclerosis.

Yasuyuki Ohta, Emi Nomura, Shinae Kizaka-Kondoh, Koji Abe

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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
1.3field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 4 institutions in 1 country.

Yasuyuki OhtaDivision of Neurology and Clinical Neuroscience, Department of Internal Medicine III, Yamagata University School of Medicine, Yamagata, Japan. yasuyuki@med.id.yamagata-u.ac.jp.
Emi NomuraDepartment of Neurology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.
Shinae Kizaka-KondohSchool of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Japan.
Koji AbeNational Center of Neurology and Psychiatry (NCNP), Kodaira city, Tokyo, Japan.
National Center of Neurology and Psychiatry · JPOkayama University · JPTokyo Institute of Technology · JPYamagata University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Oxidative and hypoxic stresses are associated with the degeneration of both motor neurons and skeletal muscles in amyotrophic lateral sclerosis (ALS). In vivo bioluminescent imaging is used to monitor cellular responses to oxidative and hypoxic stresses in living ALS model mice bearing G93A-human Cu/Zn superoxide dismutase (SOD1) longitudinally using the IVIS spectrum imaging system. Double transgenic mice bearing both Keap1-dependent oxidative stress detector No-48 (OKD48) and G93A-SOD1 are useful for in vivo imaging of oxidative stress in ALS. We developed a bioluminescence resonance energy transfer (BRET) probe that is regulated by HIF-1α-specific ubiquitin-proteasome system. G93A-SOD1 mice injected with the BRET probe are useful to investigate the spatiotemporal responses to hypoxic stress in ALS. In this chapter, we introduce a practical protocol of in vivo imaging of both oxidative and hypoxic stress in ALS model mice.

Indexed as

Amyotrophic Lateral SclerosisAnimalsDisease Models, AnimalHumansHypoxiaKelch-Like ECH-Associated Protein 1MiceMice, TransgenicNF-E2-Related Factor 2Oxidative StressSuperoxide DismutaseSuperoxide Dismutase-1Kelch-Like ECH-Associated Protein 1NF-E2-Related Factor 2Superoxide DismutaseSuperoxide Dismutase-1Amyotrophic lateral sclerosis (ALS)Bioluminescence resonance energy transfer (BRET)Hypoxia inducible factor-1 (HIF-1)Hypoxic stressIn vivo imagingNuclear erythroid 2-related factor 2 (Nrf2)Oxidative stressSuperoxide dismutase (SOD1)

Identifiers

PMID35836077
OpenAlexW4285413964

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.