ArticleThe Journal of biological chemistry2022
Profiling 26,000 Aplysia californica neurons by single cell mass spectrometry reveals neuronal populations with distinct neuropeptide profiles.
Article in The Journal of biological chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 21 citations in OpenAlex.
- Protocol for reconstructing 2D and 3D peptide atlases of the Ciona cerebral ganglion at single-cell resolution.STAR protocols · 2026Article
- Spatially Mapping Neuropeptide Isomers via MALDI Trapped Ion Mobility MS Imaging.Analytical chemistry · 2025Article
- Two- and three-dimensional neuropeptidomic landscape in the central nervous system of an invertebrate chordate,iScience · 2025Article
- Fluorescence-Guided Matrix-assisted Laser Desorption/Ionization with Laser-Induced Postionization Mass Spectrometry of Individual Rat Neural Cells.Journal of visualized experiments : JoVE · 2025Article
- Decoding Neuropeptide Complexity: Advancing Neurobiological Insights from Invertebrates to Vertebrates through Evolutionary Perspectives.ACS chemical neuroscience · 2025Review
- Single-Cell Peptide Profiling to Distinguish Stickleback Ecotypes with Divergent Breeding Behavior.Journal of proteome research · 2025Article
- Mass-Guided Single-Cell MALDI Imaging of Low-Mass Metabolites Reveals Cellular Activation Markers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Neurochemical and Morphological Comparisons of Motor Nerve Organoids and Spinal-Cord Explants.ACS chemical neuroscience · 2025Article
- Single Cell mass spectrometry: Towards quantification of small molecules in individual cells.Trends in analytical chemistry : TRAC · 2024Article
- Persistent modulatory actions and task switching in the feeding network of Aplysia.Current opinion in neurobiology · 2023Review
- Identification of three elevenin receptors and roles of elevenin disulfide bond and residues in receptor activation in Aplysia californica.Scientific reports · 2023Article
- Chemical Decrosslinking-Based Peptide Characterization of Formaldehyde-Fixed Rat Pancreas Using Fluorescence-Guided Single-Cell Mass Spectrometry.Analytical chemistry · 2023Article
- Uncovering biology by single-cell proteomics.Communications biology · 2023Review
- Peptidomics.Nature reviews. Methods primers · 2023Article
- Data-Driven and Machine Learning-Based Framework for Image-Guided Single-Cell Mass Spectrometry.Journal of proteome research · 2023Article
- Characterization of anFrontiers in pharmacology · 2023Article
- Recent Advancements in the Characterization of D-Amino Acid and Isoaspartate Post-Translational Modifications.Mass spectrometry reviewsReview
- Distribution, cellular localization, and colocalization of several peptide neurotransmitters in the central nervous system ofLearning & memory (Cold Spring Harbor, N.Y.)Article
- Probe-based mass spectrometry approaches for single-cell and single-organelle measurements.Mass spectrometry reviewsReview
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Neuropeptides are a chemically diverse class of cell-to-cell signaling molecules that are widely expressed throughout the central nervous system, often in a cell-specific manner. While cell-to-cell differences in neuropeptides is expected, it is often unclear how exactly neuropeptide expression varies among neurons. Here we created a microscopy-guided, high-throughput single cell matrix-assisted laser desorption/ionization mass spectrometry approach to investigate the neuropeptide heterogeneity of individual neurons in the central nervous system of the neurobiological model Aplysia californica, the California sea hare. In all, we analyzed more than 26,000 neurons from 18 animals and assigned 866 peptides from 66 prohormones by mass matching against an in silico peptide library generated from known Aplysia prohormones retrieved from the UniProt database. Louvain-Jaccard (LJ) clustering of mass spectra from individual neurons revealed 40 unique neuronal populations, or LJ clusters, each with a distinct neuropeptide profile. Prohormones and their related peptides were generally found in single cells from ganglia consistent with the prohormones' previously known ganglion localizations. Several LJ clusters also revealed the cellular colocalization of behaviorally related prohormones, such as an LJ cluster exhibiting achatin and neuropeptide Y, which are involved in feeding, and another cluster characterized by urotensin II, small cardiac peptide, sensorin A, and FRFa, which have shown activity in the feeding network or are present in the feeding musculature. This mass spectrometry-based approach enables the robust categorization of large cell populations based on single cell neuropeptide content and is readily adaptable to the study of a range of animals and tissue types.
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