ArticleCellular and molecular life sciences : CMLS2022
Molecular crowding facilitates bundling of IMPDH polymers and cytoophidium formation.
Article in Cellular and molecular life sciences : CMLS, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- The Impact of Induction of Insulin Secretion in MIN6 Cells on the Extent of Cytoophidia Formation and Their Structural Forms.Iranian journal of biotechnology · 2026Article
- Structural basis for a filamentous morpheein model of human cystathionine beta-synthase.Nature communications · 2026Article
- Beyond hepatitis C: clinical spectrum and demographic characteristics of anti-rods and rings antibodies in a large ANA-positive cohort.Frontiers in immunology · 2026Article
- Enzyme Assemblies in Nucleotide Metabolism: Structure, Regulation, and Disease Implications.Advances in experimental medicine and biology · 2026Review
- Y12C mutation disrupts IMPDH cytoophidia and alters cancer metabolism.The FEBS journal · 2025Article
- Epstein-Barr virus latent membrane protein 1 subverts IMPDH pathways to drive B-cell oncometabolism.PLoS pathogens · 2025Article
- Differential Cytoophidium Assembly betweenInternational journal of molecular sciences · 2024Article
- The IMPDH cytoophidium couples metabolism and fetal development in mice.Cellular and molecular life sciences : CMLS · 2024Article
- Dynamic Cytoophidia during Late-StageInternational journal of molecular sciences · 2024Article
- Cytoophidia Influence Cell Cycle and Size inInternational journal of molecular sciences · 2024Article
- A journey into the regulatory secrets of theFrontiers in pharmacology · 2024Review
- A model industrial workhorse: Bacillus subtilis strain 168 and its genome after a quarter of a century.Microbial biotechnology · 2023Review
- Structural basis of human PRPS2 filaments.Cell & bioscience · 2023Article
- Greater than the sum of parts: Mechanisms of metabolic regulation by enzyme filaments.Current opinion in structural biology · 2023Review
- Effect on cell survival and cytoophidium assembly of the adRP-10-related IMPDH1 missense mutation Asp226Asn.Frontiers in cell and developmental biology · 2023Article
- Super-Resolution Imaging Reveals Dynamic Reticular Cytoophidia.International journal of molecular sciences · 2022Article
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Authors and funding
7 authors.
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Abstract
The cytoophidium is a unique type of membraneless compartment comprising of filamentous protein polymers. Inosine monophosphate dehydrogenase (IMPDH) catalyzes the rate-limiting step of de novo GTP biosynthesis and plays critical roles in active cell metabolism. However, the molecular regulation of cytoophidium formation is poorly understood. Here we show that human IMPDH2 polymers bundle up to form cytoophidium-like aggregates in vitro when macromolecular crowders are present. The self-association of IMPDH polymers is suggested to rely on electrostatic interactions. In cells, the increase of molecular crowding with hyperosmotic medium induces cytoophidia, while the decrease of that by the inhibition of RNA synthesis perturbs cytoophidium assembly. In addition to IMPDH, CTPS and PRPS cytoophidium could be also induced by hyperosmolality, suggesting a universal phenomenon of cytoophidium-forming proteins. Finally, our results indicate that the cytoophidium can prolong the half-life of IMPDH, which is proposed to be one of conserved functions of this subcellular compartment.
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