Evidence map›Paper›PMID 35833801›Full record

ArticleThe CRISPR journal2022

Cas12a-Capture: A Novel, Low-Cost, and Scalable Method for Targeted Sequencing.

Taylor L Mighell, Andrew Nishida, Brendan L O'Connell, Caitlin V Miller, Sally Grindstaff, Casey A Thornton, Andrew C Adey, Daniel Doherty, Brian J O'Roak

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The CRISPR journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Taylor L MighellDepartment of Molecular & Medical Genetics, Oregon Health & Science University, Portland, Oregon, USA; Portland, Oregon, USA.
Andrew NishidaDepartment of Molecular & Medical Genetics, Oregon Health & Science University, Portland, Oregon, USA; Portland, Oregon, USA.
Brendan L O'ConnellDepartment of Molecular & Medical Genetics, Oregon Health & Science University, Portland, Oregon, USA; Portland, Oregon, USA.
Caitlin V MillerDepartment of Pediatrics, University of Washington, Seattle, Washington, USA; and Portland, Oregon, USA.
Sally GrindstaffDepartment of Molecular & Medical Genetics, Oregon Health & Science University, Portland, Oregon, USA; Portland, Oregon, USA.
Casey A ThorntonDepartment of Molecular & Medical Genetics, Oregon Health & Science University, Portland, Oregon, USA; Portland, Oregon, USA.
Andrew C AdeyDepartment of Molecular & Medical Genetics, Oregon Health & Science University, Portland, Oregon, USA; Portland, Oregon, USA.
Daniel DohertyDepartment of Pediatrics, University of Washington, Seattle, Washington, USA; and Portland, Oregon, USA.
Brian J O'RoakDepartment of Molecular & Medical Genetics, Oregon Health & Science University, Portland, Oregon, USA; Portland, Oregon, USA.ORCID 0000-0002-4141-0095

Funding

Intellectual and Developmental Disabilities Research CenterU54HD083091 · NICHD · UNIVERSITY OF WASHINGTON · PI MURPHY, SEAN PADRAIG · 2014 to 2019
$7.9M
Identifying the missing heritability in recessive disorders using Joubert syndrome as a modelR01HD100730 · NICHD · UNIVERSITY OF WASHINGTON · PI DOHERTY, DANIEL · 2020 to 2023
$2.0M
NICHD NIH HHS R01 HD100730NICHD NIH HHS U54 HD083091
6 · The paper itself

Abstract

Targeted sequencing remains a valuable technique for clinical and research applications. However, many existing technologies suffer from pervasive guanine-cytosine (GC) sequence content bias, high input DNA requirements, and high cost for custom panels. We have developed Cas12a-Capture, a low-cost and highly scalable method for targeted sequencing. The method utilizes preprogrammed guide RNAs to direct CRISPR-Cas12a cleavage of double-stranded DNA

Indexed as

CRISPR-Cas SystemsGene EditingDNANucleotidesRNA, Guide, CRISPR-Cas SystemsDNANucleotidesRNA, Guide, CRISPR-Cas Systems

Identifiers

PMID35833801
PMCPMC9419982

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.