Evidence map›Paper›PMID 35833800›Full record

ArticleThe CRISPR journal2022

Adaptation by Type V-A and V-B CRISPR-Cas Systems Demonstrates Conserved Protospacer Selection Mechanisms Between Diverse CRISPR-Cas Types.

Wen Y Wu, Simon A Jackson, Cristóbal Almendros, Anna C Haagsma, Suzan Yilmaz, Gerrit Gort, John van der Oost, Stan J J Brouns, Raymond H J Staals

Abstract read
In one paragraph

Article in The CRISPR journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Molecular mechanisms and biotechnology applications of CRISPR-Cas12a.Nature reviews. Molecular cell biology · 2026
    Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Wen Y WuLaboratory of Microbiology, Wageningen University and Research, Wageningen, The Netherlands; Wageningen University and Research, Wageningen, The Netherlands.
Simon A JacksonDepartment of Microbiology and Immunology, University of Otago, Dunedin, New Zealand; Wageningen University and Research, Wageningen, The Netherlands.
Cristóbal AlmendrosDepartment of Bionanoscience, Delft University of Technology, Delft, The Netherlands; Wageningen University and Research, Wageningen, The Netherlands.
Anna C HaagsmaDepartment of Bionanoscience, Delft University of Technology, Delft, The Netherlands; Wageningen University and Research, Wageningen, The Netherlands.
Suzan YilmazLaboratory of Microbiology, Wageningen University and Research, Wageningen, The Netherlands; Wageningen University and Research, Wageningen, The Netherlands.ORCID 0000-0001-7511-6073
Gerrit GortBiometris, Wageningen University and Research, Wageningen, The Netherlands.
John van der OostLaboratory of Microbiology, Wageningen University and Research, Wageningen, The Netherlands; Wageningen University and Research, Wageningen, The Netherlands.ORCID 0000-0001-5024-1871
Stan J J BrounsDepartment of Bionanoscience, Delft University of Technology, Delft, The Netherlands; Wageningen University and Research, Wageningen, The Netherlands.ORCID 0000-0002-9573-1724
Raymond H J StaalsLaboratory of Microbiology, Wageningen University and Research, Wageningen, The Netherlands; Wageningen University and Research, Wageningen, The Netherlands.ORCID 0000-0002-5741-9457

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Adaptation of clustered regularly interspaced short palindromic repeats (CRISPR) arrays is a crucial process responsible for the unique, adaptive nature of CRISPR-Cas immune systems. The acquisition of new CRISPR spacers from mobile genetic elements has previously been studied for several types of CRISPR-Cas systems. In this study, we used a high-throughput sequencing approach to characterize CRISPR adaptation of the type V-A system from

Indexed as

CRISPR-Associated ProteinsCRISPR-Cas SystemsDNAEndonucleasesGene EditingCRISPR-Associated ProteinsDNAEndonucleases

Identifiers

PMID35833800
PMCPMC9419969

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.