ReviewNature protocols2022
Isotropic imaging across spatial scales with axially swept light-sheet microscopy.
Review in Nature protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed.
- ONE microscopy.Nature protocols · 2026Review
- Polarization-engineered aberration-resilient light sheet microscopy.bioRxiv : the preprint server for biology · 2026Article
- Whole organism 3D mapping reveals universal branching topology and biophysical optimization governs vascular and nervous system development.bioRxiv : the preprint server for biology · 2026Article
- pan-ASLM: Axially Swept Light Sheet Microscopy for Fast and High-Resolution Imaging of Expanded Samples.Npj imaging · 2026Article
- Article
- Mechanical de-skewing enables high-resolution imaging of thin tissue slices with a mesoSPIM light-sheet microscope.Biomedical optics express · 2026Article
- Axially Swept Light-Sheet Microscopy using scattering and fluorescence contrast mechanisms.Proceedings of SPIE--the International Society for Optical Engineering · 2026Article
- Rapid and gentle volumetric imaging of host-pathogen interactions in salmon skin cells using projective oblique plane microscopy.Scientific reports · 2025Article
- Altair-LSFM A High-Resolution, Easy-to-Build Light-Sheet Microscope for Sub-Cellular Imaging.bioRxiv : the preprint server for biology · 2025Article
- Universal consensus 3D segmentation of cells from 2D segmented stacks.Nature methods · 2025Article
- Refractive Index-Corrected Light-Sheet Microscopy for Macro-View Cardiovascular Imaging.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Feature-driven whole-tissue imaging with subcellular resolution.Cell reports methods · 2025Article
- The Vps13-like protein BLTP2 regulates phosphatidylethanolamine levels to maintain plasma membrane fluidity and breast cancer aggressiveness.Nature cell biology · 2025Article
- A parallelly distributed microscope and software system for scalable high-throughput multispectral 3D imaging.bioRxiv : the preprint server for biology · 2025Article
- Ring deconvolution microscopy: exploiting symmetry for efficient spatially varying aberration correction.Nature methods · 2025Article
- A multiwell plate approach to increase the sample throughput during tissue clearing.Nature protocols · 2025Review
- Imaging of cellular dynamics from a whole organism to subcellular scale with self-driving, multiscale microscopy.Nature methods · 2025Article
- Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties.Nature cell biology · 2025Article
- System- and sample-agnostic isotropic three-dimensional microscopy by weakly physics-informed, domain-shift-resistant axial deblurring.Nature communications · 2025Article
- Mesoscopic axially swept oblique plane microscope for the imaging of freely moving organisms with near-isotropic resolution.Biomedical optics express · 2024Article
Corrections and comments
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Authors and funding
13 authors.
Funding
Abstract
Light-sheet fluorescence microscopy is a rapidly growing technique that has gained tremendous popularity in the life sciences owing to its high-spatiotemporal resolution and gentle, non-phototoxic illumination. In this protocol, we provide detailed directions for the assembly and operation of a versatile light-sheet fluorescence microscopy variant, referred to as axially swept light-sheet microscopy (ASLM), that delivers an unparalleled combination of field of view, optical resolution and optical sectioning. To democratize ASLM, we provide an overview of its working principle and applications to biological imaging, as well as pragmatic tips for the assembly, alignment and control of its optical systems. Furthermore, we provide detailed part lists and schematics for several variants of ASLM that together can resolve molecular detail in chemically expanded samples, subcellular organization in living cells or the anatomical composition of chemically cleared intact organisms. We also provide software for instrument control and discuss how users can tune imaging parameters to accommodate diverse sample types. Thus, this protocol will serve not only as a guide for both introductory and advanced users adopting ASLM, but as a useful resource for any individual interested in deploying custom imaging technology. We expect that building an ASLM will take ~1-2 months, depending on the experience of the instrument builder and the version of the instrument.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.