Evidence map›Paper›PMID 35829815›Full record

ReviewHistochemistry and cell biology2022

Three-dimensional SEM, TEM, and STEM for analysis of large-scale biological systems.

Snježana Radulović, Sowmya Sunkara, Reinhard Rachel, Gerd Leitinger

Open access · hybridAbstract readReview
In one paragraph

Review in Histochemistry and cell biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
4.1field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 21 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. In focus in Vienna: Microscopy and cellular organelles.Histochemistry and cell biology · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Snježana RadulovićDivision of Cell Biology, Histology and Embryology, Research Unit Electron Microscopic Techniques, Gottfried Schatz Research Center, Medical University of Graz, 8010, Graz, Austria.
Sowmya SunkaraDivision of Cell Biology, Histology and Embryology, Research Unit Electron Microscopic Techniques, Gottfried Schatz Research Center, Medical University of Graz, 8010, Graz, Austria.
Reinhard RachelCentre for Electron Microscopy, University of Regensburg, 93053, Regensburg, Germany.
Gerd LeitingerDivision of Cell Biology, Histology and Embryology, Research Unit Electron Microscopic Techniques, Gottfried Schatz Research Center, Medical University of Graz, 8010, Graz, Austria. gerd.leitinger@medunigraz.at.
Medical University of Graz · ATUniversity of Regensburg · DE

Funding

Austrian Science Fund P 32058 and P 29370Austrian Science Fund FWF P 29370Austrian Science Fund FWF P 32058
6 · The paper itself

Abstract

A major aim in structural cell biology is to analyze intact cells in three dimensions, visualize subcellular structures, and even localize proteins at the best possible resolution in three dimensions. Though recently developed electron microscopy tools such as electron tomography, or three-dimensional (3D) scanning electron microscopy, offer great resolution in three dimensions, the challenge is that, the better the resolution, usually the smaller the volume under investigation. Several different approaches to overcome this challenge were presented at the Microscopy Conference in Vienna in 2021. These tools include array tomography, batch tomography, or scanning transmission electron tomography, all of which can nowadays be extended toward correlative light and electron tomography, with greatly increased 3D information. Here, we review these tools, describe the underlying procedures, and discuss their advantages and limits.

Indexed as

Electron Microscope TomographyImaging, Three-DimensionalMicroscopy, Electron, ScanningMicroscopy, Electron, Scanning Transmission3D reconstructionArray tomographyCorrelative light and electron microscopyElectron tomographyLarge volumesStructural cell biology

Identifiers

PMID35829815
PMCPMC9399040
OpenAlexW4285089426

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.