Evidence map›Paper›PMID 35821489›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Dual-Luciferase-Based Fast and Sensitive Detection of Malaria Hypnozoites for the Discovery of Anti-relapse Compounds.

Annemarie M Voorberg-van der Wel, Anne-Marie Zeeman, Ivonne G Nieuwenhuis, Nicole M van der Werff, Clemens H M Kocken

Open access · greenAbstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Transfection Models to InvestigatePathogens (Basel, Switzerland) · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Annemarie M Voorberg-van der WelDepartment of Parasitology, Biomedical Primate Research Centre, Rijswijk, The Netherlands.
Anne-Marie ZeemanDepartment of Parasitology, Biomedical Primate Research Centre, Rijswijk, The Netherlands.
Ivonne G NieuwenhuisDepartment of Parasitology, Biomedical Primate Research Centre, Rijswijk, The Netherlands.
Nicole M van der WerffDepartment of Parasitology, Biomedical Primate Research Centre, Rijswijk, The Netherlands.
Clemens H M KockenDepartment of Parasitology, Biomedical Primate Research Centre, Rijswijk, The Netherlands. kocken@bprc.nl.
Biomedical Primate Research Centre · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Malaria hypnozoites are dormant parasite stages that reside inside hepatocytes. Upon activation, these stages can resume growth, causing new episodes of blood stage malaria infection. This chapter describes a fast and sensitive protocol for the detection of bioluminescent (BL) hypnozoites in vitro. Using transgenic Plasmodium cynomolgi parasites that differentially express the BL reporter proteins firefly luciferase and the ultrabright NanoLuc, hypnozoites can be distinguished from liver stage schizonts. This robust method sets the stage for implementation in large-scale drug screening platforms with the aim to find new compounds that eliminate hypnozoites.

Indexed as

MalariaPlasmodium cynomolgiHepatocytesHumansLuciferasesRecurrenceLuciferasesnanolucBioluminescence (BL)Drug screeningDualFirefly luciferaseHepatocytesHypnozoitesLiverLiver stagesMalariaMonkeysNanoLucPlasmodium cynomolgiSporozoites

Identifiers

PMID35821489
OpenAlexW4285256880

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.