Evidence map›Paper›PMID 35821488›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Longitudinal Tracing of Lyssavirus Infection in Mice via In Vivo Bioluminescence Imaging.

Kate E Mastraccio, Celeste Huaman, Eric D Laing, Christopher C Broder, Brian C Schaefer

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 citations in OpenAlex.

  1. Article
  2. AnFrontiers in chemical biology · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Kate E Mastraccio *Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA.
Celeste Huaman *Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA.
Eric D Laing *Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA.
Christopher C Broder *Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA.
Brian C SchaeferDepartment of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA. Brian.Schaefer@usuhs.edu.
Uniformed Services University of the Health Sciences · USWadsworth Center · US

Funding

T cell receptor-activated autophagy as a regulator of T cell effector responsesR01AI125552 · NIAID · HENRY M. JACKSON FDN FOR THE ADV MIL/MED · PI CONRADS, THOMAS PATRICK, HE, YOU-WEN · 2017 to 2021
$3.1M
Elucidating TCR signaling mechanisms via quantitative pattern analysis_administrative supplementU01GM109887 · NIGMS · HENRY M. JACKSON FDN FOR THE ADV MIL/MED · PI LOSERT, WOLFGANG, SCHAEFER, BRIAN · 2014 to 2017
$1.1M
NIAID NIH HHS R01 AI125552NIGMS NIH HHS U01 GM109887
6 · The paper itself

Abstract

Bioluminescence imaging (BLI) is a technique that can be employed to quantify biological processes in living cells. When used in small animal models such as mice, BLI can provide both longitudinal and positional information regarding the biological process under investigation. Although perhaps best known for its utility in non-invasively quantifying tumor burden over time in experimental animals, BLI has also been applied in many pathogenesis models to track pathogen burden and responses to therapeutic interventions. In this chapter, we present a BLI-based method for tracing anatomical progression of lyssavirus infection in a mouse model. We also include validation methods to ensure that semiquantitative BLI data correlate well with viral load. Due to the longitudinal nature of this approach, lyssavirus pathogenesis and therapeutic intervention studies can be performed with far fewer animals than more traditional approaches, which typically require euthanasia of large animal groups at every data collection time point.

Indexed as

Luminescent MeasurementsLyssavirusAnimalsDiagnostic ImagingDisease Models, AnimalMiceAustralian bat lyssavirus (ABLV)Bioluminescence imaging (BLI)In vivo imagingLongitudinal studiesLuciferaseLyssavirusMiceRabies

Identifiers

PMID35821488
OpenAlexW4285297403

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.