Evidence map›Paper›PMID 35821476›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Bioluminescent and Fluorescent Reporter-Expressing Recombinant SARS-CoV-2.

Desarey Morales Vasquez, Kevin Chiem, Chengjin Ye, Luis Martinez-Sobrido

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. A bireporter recombinant SARS-CoV-2 Omicron BA.5 forbioRxiv : the preprint server for biology · 2026
    Article
  3. Article
  4. Bacterial Artificial Chromosome Reverse Genetics Approaches for SARS-CoV-2.Methods in molecular biology (Clifton, N.J.) · 2024
    Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Desarey Morales VasquezTexas Biomedical Research Institute, San Antonio, TX, USA.
Kevin ChiemTexas Biomedical Research Institute, San Antonio, TX, USA.
Chengjin YeTexas Biomedical Research Institute, San Antonio, TX, USA.
Luis Martinez-SobridoTexas Biomedical Research Institute, San Antonio, TX, USA. lmartinez@txbiomed.org.

Funding

NIAID Centers of Excellence for Influenza Research and Response: Universal Influenza Vaccine Research Activities75N93021C00014 · NIAID · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI GARCIA-SASTRE, ADOLFO · 2021 to 2025
$62.6M
Mechanism-based Targeting of the RNA Processing Machinery of SARS-CoV-2R01AI161363 · NIAID · UNIVERSITY OF TEXAS HLTH SCIENCE CENTER · PI GUPTA, YOGESH K · 2021 to 2025
$3.2M
The origin and future protective activity of SARS-CoV-2 RBD specific neutralizing antibodiesR01AI161175 · NIAID · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI KOBIE, JAMES J, MARTINEZ-SOBRIDO, LUIS · 2021 to 2024
$2.5M
Developing a Thermostable SARS-CoV-2 RBD-particle VaccineR43AI165089 · NIAID · POP BIOTECHNOLOGIES, INC · PI HUANG, WEI-CHIAO · 2021 to 2022
$600k
NIAID NIH HHS 75N93021C00014NIAID NIH HHS R01 AI161175NIAID NIH HHS R01 AI161363NIAID NIH HHS R43 AI165089
6 · The paper itself

Abstract

Reporter-expressing recombinant severe acute respiratory syndrome coronavirus 2 (rSARS-CoV-2) represents an excellent tool to understand the biology of and ease studying viral infections in vitro and in vivo. The broad range of applications of reporter-expressing recombinant viruses is due to the facilitated expression of fluorescence or bioluminescence readouts. In this chapter, we describe a detailed protocol on the generation of rSARS-CoV-2 expressing Venus, mCherry, and NLuc that represents a valid surrogate to track viral infections.

Indexed as

COVID-19SARS-CoV-2HumansImmunologic TestsRespiratory SystemBioluminescence (BL)CoronavirusCoronavirus disease 2019 (COVID-19)Fluorescence (FL)mCherryNanoLuc (NLuc)Recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Reporter genesReporter virusVenus

Identifiers

PMID35821476
PMCPMC10243362

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.