ArticleGenome biology2022
An ORFeome of rice E3 ubiquitin ligases for global analysis of the ubiquitination interactome.
Article in Genome biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 67 citations in OpenAlex.
- E3 ubiquitin ligases in plant defense: mechanisms and prospects for resistance breeding.Science China. Life sciences · 2026Review
- From Defense Executor to Engineering Target: Harnessing Lignin for Crop Resistance.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- The Rice E3 Ubiquitin Ligase Gene OsPUB77 Regulates Head Milled Rice Rate by Affecting Grain Starch Accumulation.Plant biotechnology journal · 2026Article
- A Conserved Magnaporthe oryzae Effector Counteracts the Rice Ubiquitin-Proteasome System by Disrupting the E2 Function to Suppress Immunity.Plant biotechnology journal · 2026Article
- A phosphorylation-dependent ubiquitination switch orchestrates nuclear immune reprogramming upon chitin perception.Nature communications · 2026Article
- Protein Post-Translational Modifications in Plant Abiotic Stress Responses.Plants (Basel, Switzerland) · 2025Review
- Mutagenesis of OsNRAMP5 Affects Blast Resistance Through Mn Absorption in Rice.Rice (New York, N.Y.) · 2025Article
- A new E3 ligase-mediated plant immune signaling pathway.Science China. Life sciences · 2025Article
- RING-Type E3 Ligase OsRFPH2-6 Ubiquitinates Nuclear Factor Y Subunit OsNF-YA1 to Suppress Rice Immunity.Molecular plant pathology · 2025Article
- Balancing rice blast resistance and growth through suppression of the E3 ubiquitin ligase OsRING80.Plant physiology · 2025Article
- Article
- A Blast-Resistant NLR Gene Confers Drought Resistance by Competitively Interacting with an E3 Ligase to Protect Phenylalanine Ammonia-Lyase in Rice.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Natural Allelic Variations in IbCHYR1-IbZnFR Complex Regulate Fusarium Root Rot Resistance in Sweet Potato.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Research Progress on Rice-Blast-Resistance-Related Genes.Plants (Basel, Switzerland) · 2025Review
- An mNature communications · 2025Article
- Article
- Global Protein Interactome Mapping in Rice Using Barcode-Indexed PCR Coupled with HiFi Long-Read Sequencing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- The Role of E3 Ubiquitin Ligase GeneInternational journal of molecular sciences · 2025Review
- Ubiquitination of OsCSN5 by OsPUB45 activates immunity by modulating the OsCUL3a-OsNPR1 module.Science advances · 2025Article
- OsLB2.2 negatively regulates rice disease resistance at seedling stage in rice.Frontiers in plant science · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
27 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundUbiquitination is essential for many cellular processes in eukaryotes, including 26S proteasome-dependent protein degradation, cell cycle progression, transcriptional regulation, and signal transduction. Although numerous ubiquitinated proteins have been empirically identified, their cognate ubiquitin E3 ligases remain largely unknown.
resultsHere, we generate a complete ubiquitin E3 ligase-encoding open reading frames (UbE3-ORFeome) library containing 98.94% of the 1515 E3 ligase genes in the rice (Oryza sativa L.) genome. In the test screens with four known ubiquitinated proteins, we identify both known and new E3s. The interaction and degradation between several E3s and their substrates are confirmed in vitro and in vivo. In addition, we identify the F-box E3 ligase OsFBK16 as a hub-interacting protein of the phenylalanine ammonia lyase family OsPAL1-OsPAL7. We demonstrate that OsFBK16 promotes the degradation of OsPAL1, OsPAL5, and OsPAL6. Remarkably, we find that overexpression of OsPAL1 or OsPAL6 as well as loss-of-function of OsFBK16 in rice displayed enhanced blast resistance, indicating that OsFBK16 degrades OsPALs to negatively regulate rice immunity.
conclusionsThe rice UbE3-ORFeome is the first complete E3 ligase library in plants and represents a powerful proteomic resource for rapid identification of the cognate E3 ligases of ubiquitinated proteins and establishment of functional E3-substrate interactome in plants.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.