Evidence map›Paper›PMID 35819774›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Generating Custom Pooled CRISPR Libraries for Genetic Dissection of Biological Pathways.

Daniel R Gulbranson

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.0field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author at 1 institution in 1 country.

Daniel R GulbransonGladstone Institute of Neurological Disease, Gladstone Institutes, San Francisco, CA, USA. daniel.gulbranson@gladstone.ucsf.edu.
Gladstone Institutes · US

Funding

Evaluation and Disruption of Tau - GSK3β Vicious CycleF32AG062039 · NIA · J. DAVID GLADSTONE INSTITUTES · PI GULBRANSON, DANIEL · 2019 to 2021
$155k
NIA NIH HHS F32 AG062039
6 · The paper itself

Abstract

Genetic screens are a classic approach to dissecting biological pathways including membrane trafficking. Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 have enabled the utility of this approach in diploid models, including cultured mammalian cells. Here, we present detailed protocols for generating custom CRISPR libraries. These methods are useful for generating genome-wide libraries for new model organisms that lack an existing genome-wide library, and for generating smaller focused libraries.

Indexed as

CRISPR-Cas SystemsGenetic TestingAnimalsCell LineGenomic LibraryMammalsCRISPR libraryCRISPR screenCustom libraryGenetic screenPooled screensgRNA cloning

Identifiers

PMID35819774
OpenAlexW4285079156

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.