Evidence map›Paper›PMID 35819766›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Localizing Proteins on Single Trafficking Organelles in 3D with Semisynthetic Gold Labeling and Platinum Replica Electron Microscopy.

Bijeta Prasai, Justin W Taraska

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 82% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

Bijeta PrasaiBiochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
Justin W TaraskaBiochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA. justin.taraska@nih.gov.
National Heart Lung and Blood Institute · USNational Institutes of Health · US

Funding

Architecture and control of vesicle fusion in excitable cellsZIAHL006098 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI TARASKA, JUSTIN · 2011 to 2025
$25.5M
Intramural NIH HHS ZIA HL006098
6 · The paper itself

Abstract

The three-dimensional structures of organelles can be visualized at high resolutions using electron microscopy and tomography. Combining genetically encoded tags with tomography enables the specific targeting and detection of identified proteins inside cells. Here, we describe a method for attaching metal-binding gold nanoparticles to proteins genetically tagged with hexa-histidine sequences. We apply this strategy to visualize the position of intracellular proteins on single organelles in unroofed cells with platinum replica electron microscopy at the nanoscale in three dimensions. We have found that this combined method can label and localize proteins with isotropic high precision to generate quantitative maps of protein positions in and around trafficking organelles at the inner plasma membrane of mammalian cells.

Indexed as

GoldMetal NanoparticlesAnimalsMammalsMicroscopy, ElectronOrganellesPlatinumGoldPlatinumCorrelative fluorescenceElectron microscopyElectron tomogramGold nanoprobeHistidine tagsPlatinum replicaPREMUnroofing

Identifiers

PMID35819766
PMCPMC9639615
OpenAlexW4285078761

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.