ArticleMethods in molecular biology (Clifton, N.J.)2022
Electron Tomographic Methods for Studying Organelles of the Murine Chemical Synapse.
Tamara Basta, Garry P Morgan, Eileen T O'Toole, Nalini R Rao, Jeffrey N Savas, Michael H B Stowell
Open access · greenAbstract read
In one paragraphArticle in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registryThe trial behind it
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4 · The recordCorrections and comments
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5 · Who and what moneyAuthors and funding
6 authors at 2 institutions in 1 country.
Tamara BastaDepartment of Molecular, Cellular & Developmental Biology, University of Colorado, Boulder, CO, USA.
Garry P MorganBoulder Electron Microscopy Services, University of Colorado, Boulder, CO, USA.
Eileen T O'TooleBoulder Electron Microscopy Services, University of Colorado, Boulder, CO, USA.
Nalini R RaoDepartment of Neurology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
Jeffrey N SavasDepartment of Neurology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA. jeffrey.savas@northwestern.edu.
Michael H B StowellDepartment of Molecular, Cellular & Developmental Biology, University of Colorado, Boulder, CO, USA. stowellm@colorado.edu.
University of Colorado Boulder · USNorthwestern University · US
Funding
PREDOCTORAL AND POSTDOCTORAL TRAINING PROGRAM IN AGING AND DEMENTIAT32AG020506 · NIA · NORTHWESTERN UNIVERSITY AT CHICAGO · PI ROBERT J VASSAR, SANDRA WEINTRAUB · 2002 to 2026
$9.9MStructural characterization of A-beta strain variation in AD mouse modelsR01AG061865 · NIA · UNIVERSITY OF SOUTHERN CALIFORNIA · PI LANGEN, RALF, SIEMER, ANSGAR B · 2018 to 2022
$4.2MUnderstanding Amyloid Pathology - Multiomic Activity Imaging of Plaque Formation Dynamics (AmyMAP)R01AG078796 · NIA · NORTHWESTERN UNIVERSITY AT CHICAGO · PI Jeffrey Nicholas Savas · 2022 to 2026
$2.7MThe role of activity induced exosome signaling in synaptic pathology of Alzheimer's DiseaseR01AG061787 · NIA · NORTHWESTERN UNIVERSITY AT CHICAGO · PI SAVAS, JEFFREY NICHOLAS · 2018 to 2022
$2.3MDefining The Impaired Proteostasis Network in ALS Patient Motor NeuronsR21NS107761 · NINDS · NORTHWESTERN UNIVERSITY AT CHICAGO · PI KISKINIS, EVANGELOS, SAVAS, JEFFREY NICHOLAS · 2018 to 2019
$435kInsights into Amyloid Pathogenicity DynamicsR21AG080705 · NIA · NORTHWESTERN UNIVERSITY AT CHICAGO · PI HANRIEDER, JORG, SAVAS, JEFFREY NICHOLAS · 2023 to 2024
$401kNIA NIH HHS R01 AG061787NIA NIH HHS R01 AG061865NIA NIH HHS R01 AG078796NIA NIH HHS R21 AG080705NIA NIH HHS T32 AG020506NINDS NIH HHS R21 NS107761
6 · The paper itselfAbstract
Electron tomography of the chemical synapse provides important architectural information regarding the organization of synaptic organelles including synaptic vesicles, Nissl bodies, and early endosomes. Here, we describe methods for the preparation of select murine brain regions for high-pressure freezing, freeze substitution, and EM tomographic analysis of synaptic structures. The method uses fresh brain slices prepared using a vibratome and biopsy punches to collect specific brain regions of interest suitable for subsequent preservation and EM tomographic imaging.
Indexed as
Electron Microscope TomographyElectronsAnimalsFreeze SubstitutionMiceOrganellesSynapsesBrainElectron microscopyElectron tomographyFreeze substitutionHigh-pressure freezingMurineSubcellular organellesSynapse
Identifiers
PMID35819763
PMCPMC10115186
OpenAlexW4285078943
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