ArticleApplied biochemistry and biotechnology2022
Native SodB Overexpression of Synechocystis sp. PCC 6803 Improves Cell Growth Under Alcohol Stresses Whereas Its Gpx2 Overexpression Impacts on Growth Recovery from Alcohol Stressors.
Article in Applied biochemistry and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 5 citations in OpenAlex.
- Stress genomics of the toxigenic cyanobacteria: environmental and biotechnological perspectives.World journal of microbiology & biotechnology · 2025Review
- Functional investigation of Zur in metal ion homeostasis, motility and multiple stresses resistance in cyanobacteria Synechocystis sp. PCC 6803.Stress biology · 2025Article
- Application of Cyanobacteria as Chassis Cells in Synthetic Biology.Microorganisms · 2024Review
- Site-2 Protease Slr1821 Regulates Carbon/Nitrogen Homeostasis during Ammonium Stress Acclimation in CyanobacteriumInternational journal of molecular sciences · 2023Article
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
To overcome the limited resistance to alcohol stress, genetically engineered Synechocystis sp. PCC 6803 strains with overexpressions of genes related with the ROS detoxification system (sodB and gpx2, which encode superoxide dismutase and glutathione peroxidase, respectively) were developed. Three engineered strains including a sodB-overexpressing strain (OE + S), a gpx2-overexpressing strain (OE + G), and a sodB/gpx2-overexpressing strain (OE + SG) grew similarly as wild-type control under normal condition. When compared to wild-type control, OE + S and OE + SG strains grew faster for 4 days under 2.0% (v/v) ethanol and 0.3% (v/v) n-butanol conditions, as well as having higher chlorophyll a levels. On the other hand, the prominent growth recovery of OE + G and OE + SG was noted within 4 days in normal BG
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