ArticleJournal of agricultural and food chemistry2022
Detection of Acrylamide in Foodstuffs by Nanobody-Based Immunoassays.
Article in Journal of agricultural and food chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 39 citations in OpenAlex.
- AC Electrokinetics-Enhanced Capacitive Aptasensor for Point-of-Care Testing of Acrylamide in Coffee.Micromachines · 2026Article
- Research progress on generation, detection and control of hazards in baked foods during thermal processing.Food chemistry: X · 2025Review
- Machine learning assisted CRCA multi-color fluorescent droplet imaging for high-sensitivity simultaneous detection of Maillard hazards.iScience · 2025Article
- Pentameric nanobodies serve as a capture agent and RANbodies function as immunoprobes for the sensitive detection of Salmonella typhimurium in immunoassays.Analytical and bioanalytical chemistry · 2025Article
- A colorimetric/SERS dual-mode sensor based on ferric ion-dopamine@Au-Ag-Au Janus NPs for acrylamide determination in baked goods.Mikrochimica acta · 2025Article
- Self-Assembled PVP-Gd Composite Nanosheets via Ultrasound Synthesis for Targeted Acrylamide Sensing in Food Safety.Journal of agricultural and food chemistry · 2025Article
- A new strategy to generate nanobodies for the coumaphos based on the synthesized nanobody libraries.Food chemistry · 2024Article
- Advancements in Chemical and Biosensors for Point-of-Care Detection of Acrylamide.Sensors (Basel, Switzerland) · 2024Review
- A bispecific nanobody with high sensitivity/efficiency for simultaneous determination of carbaryl and its metabolite 1-naphthol in the soil and rice samples.Environmental pollution (Barking, Essex : 1987) · 2023Article
- Structural Insights into the Stability and Recognition Mechanism of the Antiquinalphos Nanobody for the Detection of Quinalphos in Foods.Analytical chemistry · 2023Article
- Ultrasensitive Electrochemical Immunosensors Using Nanobodies as Biocompatible Sniffer Tools of Agricultural Contaminants and Human Disease Biomarkers.Micromachines · 2023Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Acrylamide is toxic aliphatic amide formed via the Maillard reaction between asparagine and reducing sugars during thermal processing of food. Herein, a specific nanobody termed Nb-7E against the acrylamide derivative xanthyl acrylamide (XAA) was isolated from an immunized phage display library and confirmed to be able to detect acrylamide. First, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was established for acrylamide with a limit of detection (LOD) of 0.089 μg/mL and working range from 0.23 to 5.6 μg/mL. Furthermore, an enhanced electrochemical immunoassay (ECIA) was developed based on the optimized reaction conditions. The LOD was as low as 0.033 μg/mL, threefold improved compared to that of ic-ELISA, and a wider linear detection range from 0.39 to 50.0 μg/mL was achieved. The average recoveries ranged from 88.29 to 111.76% in spiked baked biscuits and potato crisps. Finally, the analytical performance of the ECIA was validated by standard ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.