ArticleMicroscopy research and technique2022
Focus variation due to near infrared laser in a confocal microscope.
Article in Microscopy research and technique, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Infrared Laser Effects on Cell Projection Depend on Irradiation Intermittence and Cell Activity.Cells · 2023Article
- Focus variation due to near infrared laser in a confocal microscope.Microscopy research and technique · 2022Article
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Authors and funding
6 authors.
Funding
Abstract
Focus precision and stability is crucial in confocal microscopy not only for image sharpness but also to avoid radiometric fluctuations that can wrongly be interpreted as variations of the fluorescence intensity in the sample. Here we report a focus variation provoked by a continuous wave laser of 810-nm wavelength introduced along the optical path of an inverted confocal microscope with an oil immersion ×60 objective. When the laser is turned on or off, the focus position drifts toward lower or high values of the vertical coordinate z, respectively. The maximum drift observed was 2.25 𝜇m for a laser power of 40 mW at the sample and over a 600-s exposure time. The temporal evolution of the focus position is well fitted by exponential curves that mimic temperature variations due to a heat source. Our analysis strongly suggests that the focus drift is due to heating of the immersion oil.
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