Evidence map›Paper›PMID 35818769›Full record

ArticleJournal of applied microbiology2022

Enhancement of Acinetobacter baumannii biofilm growth by cephem antibiotics via enrichment of protein and extracellular DNA in the biofilm matrices.

Kaoru Yamabe, Yukio Arakawa, Masaki Shoji, Katsushiro Miyamoto, Takahiro Tsuchiya, Katsuhiko Minoura, Yukihiro Akeda, Kazunori Tomono, Mitsuko Onda

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of applied microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.7field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 11 citations in OpenAlex.

  1. Antibiotic resistance dissemination in soil ecosystems: deep understanding for effective management and global health protection.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Kaoru YamabeDepartment of Social and Administrative Pharmacy, Osaka Medical and Pharmaceutical University, Takatsuki, Osaka, Japan.
Yukio ArakawaDepartment of Social and Administrative Pharmacy, Osaka Medical and Pharmaceutical University, Takatsuki, Osaka, Japan.
Masaki ShojiDepartment of Social and Administrative Pharmacy, Osaka Medical and Pharmaceutical University, Takatsuki, Osaka, Japan.
Katsushiro MiyamotoDepartment of Microbiology and Infection Control, Osaka Medical and Pharmaceutical University, Takatsuki, Osaka, Japan.
Takahiro TsuchiyaDepartment of Microbiology and Infection Control, Osaka Medical and Pharmaceutical University, Takatsuki, Osaka, Japan.
Katsuhiko MinouraJoint Research Center, Osaka Medical and Pharmaceutical University, Takatsuki, Osaka, Japan.
Yukihiro AkedaDepartment of Bacteriology, National Institute of Infectious Diseases, Tokyo, Japan.
Kazunori TomonoOsaka Institute of Public Health, Osaka, Japan.
Mitsuko OndaDepartment of Social and Administrative Pharmacy, Osaka Medical and Pharmaceutical University, Takatsuki, Osaka, Japan.ORCID https://orcid.org/0000-0002-5855-2727
Osaka University of Pharmaceutical Sciences · JPNational Institute of Infectious Diseases · JPOsaka Prefectural Institute of Public Health · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimsThe aims were to determine the effects of subinhibitory concentrations of eight cephem and carbapenem antibiotics on the biofilm formation of Acinetobacter baumannii cells and examine their effects on pre-established biofilms. METHODS AND

resultsEffects of antibiotics on biofilm formation were assayed using microtitre plates with polystyrene peg-lids. Cefmetazole, ceftriaxone, ceftazidime and cefpirome increased the biomass of pre-established biofilms on pegs in the range of their sub-minimum inhibitory concentrations (MICs), whereas none increased biofilm formation by planktonic cells. Carbapenems had a negative effect. The constituents of antibiotic-induced biofilms were analysed. Ceftriaxone or ceftazidime treatment markedly increased the matrix constituent amounts in the biofilms (carbohydrate, 2.7-fold; protein, 8.9-12.7-fold; lipid, 3.3-3.6-fold; DNA, 9.1-12.2-fold; outer membrane vesicles, 2.7-3.8-fold and viable cells, 6.8-10.1-fold). The antibiotic-enhanced biofilms had increased outer membrane protein A and were resistant to the anti-biofilm effect of azithromycin.

conclusionsSome cephems increased the biomass of pre-established biofilms in the ranges of their sub-MICs. The antibiotic-enhanced biofilms possessed more virulent characteristics than normal biofilms. SIGNIFICANCE AND IMPACT OF THE STUDY: Incomplete administration of certain cephems following biofilm-related Ac. baumannii infections could adversely cause exacerbated and chronic clinical results.

Indexed as

Acinetobacter baumanniiAnti-Bacterial AgentsCarbapenemsCeftazidimeCeftriaxoneDNAExtracellular Polymeric Substance MatrixAnti-Bacterial AgentsCarbapenemsCeftazidimeCeftriaxoneDNAAcinetobacter baumanniiazithromycinbiofilmcarbapenem antibioticscephem antibioticsouter membrane protein Aouter membrane vesicles

Identifiers

PMID35818769
PMCPMC9539989
OpenAlexW4285041403

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.