ArticleJournal of ginseng research2022
Modification of ginsenoside saponin composition via the CRISPR/Cas9-mediated knockout of protopanaxadiol 6-hydroxylase gene in
Article in Journal of ginseng research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Medicinal-plant immunity and specialized metabolism in plant-pathogen interactions: mechanisms and applications.Planta · 2026Review
- Multivariate Statistical Evaluation of Ginseng Quality and Antioxidant Activity.Molecules (Basel, Switzerland) · 2026Article
- Immunomodulatory functions of ginsenosides in skin diseases: molecular mechanisms and therapeutic prospects.Frontiers in pharmacology · 2026Review
- Harnessing Thalassochemicals: Marine Saponins as Bioactive Agents in Nutraceuticals and Food Technologies.Marine drugs · 2025Review
- Application and Progress of Genomics in Deciphering the Genetic Regulation Mechanisms of Plant Secondary Metabolites.Plants (Basel, Switzerland) · 2025Review
- Article
- CRISPR/Cas9-mediatedHorticulture research · 2025Article
- Ginsenoside Rg1 in Parkinson's disease: from basic research to clinical applications.Frontiers in pharmacology · 2025Review
- Evolution, Structural and Functional Characteristics of thePlants (Basel, Switzerland) · 2024Article
- A Novel Biosynthetic Strategy for Ginsenoside Ro: Construction of a Metabolically EngineeredInternational journal of molecular sciences · 2024Article
- A Fast, Efficient, and Tissue-Culture-Independent Genetic Transformation Method forPlants (Basel, Switzerland) · 2024Article
- Glycyrrhizin Production in Licorice Hairy Roots Based on Metabolic Redirection of Triterpenoid Biosynthetic Pathway by Genome Editing.Plant & cell physiology · 2024Article
- Application and development of CRISPR technology in the secondary metabolic pathway of the active ingredients of phytopharmaceuticals.Frontiers in plant science · 2024Review
- Regulation of transcriptome networks that mediate ginsenoside biosynthesis by essential ecological factors.PloS one · 2023Article
- Genes and Regulatory Mechanisms for Ginsenoside Biosynthesis.Journal of plant biology = Singmul Hakhoe chi · 2023Review
- Genetic manipulation of anti-nutritional factors in major crops for a sustainable diet in future.Frontiers in plant science · 2022Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: The roots of Methods: Two sgRNAs (single guide RNAs) were designed for target mutations in the exon sequences of the two PPT synthase genes (both PPTa and PPTg sequences) with the CRISPR/Cas9 system. Transgenic ginseng roots were generated through Agrobacterium-mediated transformation. The mutant lines were screened by ginsenoside analysis and DNA sequencing. Result: Ginsenoside analysis revealed the complete depletion of PPT-type ginsenosides in three putative mutant lines (Cr4, Cr7, and Cr14). The reduction of PPT-type ginsenosides in mutant lines led to increased accumulation of PPD-type ginsenosides. The gene editing in the selected mutant lines was confirmed by targeted deep sequencing. Conclusion: We have established the genome editing protocol by CRISPR/Cas9 system in
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