ArticleNature metabolism2022
Rescuing yeast from cell death enables overproduction of fatty acids from sole methanol.
Article in Nature metabolism, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 155 citations in OpenAlex.
- Iterative evolution ofSynthetic and systems biotechnology · 2026Article
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- Yeast species resource exploration and application in cell factories.Synthetic and systems biotechnology · 2026Review
- Process-integrated engineered resting cells for biocatalytic production of rare natural sugars from sole methanol molecules.Nature communications · 2026Article
- Engineering metazoan fatty acid synthase to control chain length applied in yeast.Nature chemical biology · 2026Article
- Repair of DNA and protein damages caused by formaldehyde improves methanol assimilation.Fundamental research · 2026Article
- Truncation ofSynthetic and systems biotechnology · 2026Article
- Advances in synthetic biology for engineering methylotrophic microbial cell factories.Journal of bacteriology · 2026Review
- Biosynthesis of pyomelanin from methanol with engineered Komagataella phaffii and its characterizations.Nature communications · 2026Article
- Metabolic engineering ofFrontiers in microbiology · 2026Article
- Development of precise genome editing and multi-copy integration tools inSynthetic and systems biotechnology · 2025Article
- Methanol biotransformation for the production of biodegradable plastic monomer L-lactate in yeast.Nature communications · 2025Article
- Multiscale metabolic engineering in biological lignin valorization.Innovation (Cambridge (Mass.)) · 2025Review
- ADP-ribose is a competitive inhibitor of methanol dehydrogenases from Bacillus methanolicus.The Journal of biological chemistry · 2025Article
- Combing Directed Enzyme Evolution with Metabolic Engineering to Develop Efficient Microbial Cell Factories.Chem & bio engineering · 2025Review
- High-level production of free fatty acids from lignocellulose hydrolysate by co-utilizing glucose and xylose in yeast.Synthetic and systems biotechnology · 2025Article
- Economic and sustainable revolution to facilitate one-carbon biomanufacturing.Nature communications · 2025Review
- Engineering yeast for tailored fatty acid profiles.Applied microbiology and biotechnology · 2025Review
- Advancing yeast metabolism for a sustainable single carbon bioeconomy.FEMS yeast research · 2025Review
- Integrating yeast biodiversity and machine learning for predictive metabolic engineering.FEMS yeast research · 2025Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methanol is an ideal feedstock for biomanufacturing that can be beneficial for global carbon neutrality; however, the toxicity of methanol limits the efficiency of methanol metabolism toward biochemical production. We here show that engineering production of free fatty acids from sole methanol results in cell death with decreased cellular levels of phospholipids in the methylotrophic yeast Ogataea polymorpha, and cell growth is restored by adaptive laboratory evolution. Whole-genome sequencing of the adapted strains reveals that inactivation of LPL1 (encoding a putative lipase) and IZH3 (encoding a membrane protein related to zinc metabolism) preserve cell survival by restoring phospholipid metabolism. Engineering the pentose phosphate pathway and gluconeogenesis enables high-level production of free fatty acid (15.9 g l
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.