ArticleFrontiers in cellular and infection microbiology2022
Rapid Visual Detection of Plasmodium Using Recombinase-Aided Amplification With Lateral Flow Dipstick Assay.
Article in Frontiers in cellular and infection microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Duplex Recombinase-Aided Amplification-Lateral Flow Dipstick (dRAA-LFD) Assay for New Zealand Green-Lipped Mussel Authentication.Biosensors · 2026Article
- Plasmodium malariae is an overlooked malaria parasite with emerging challenges.Communications medicine · 2026Review
- Methylene blue as a new signal tracer for nucleic acid-based lateral flow assay.Scientific reports · 2025Article
- Asymptomatic/submicroscopicParasite epidemiology and control · 2025Article
- Establishment of a Rapid Detection Technique Based on RPA-LFD and RPA-CRISPR/Cas12a onMicroorganisms · 2025Article
- Rapid visual detection of hepatitis E virus combining reverse transcription recombinase-aided amplification with lateral flow dipstick and real-time fluorescence.Journal of clinical microbiology · 2025Article
- Survey ofTransboundary and emerging diseases · 2025Article
- Establishment of reverse transcription recombinase-aided amplification with lateral flow dipstick for the rapid visual detection of Getah virus.Frontiers in cellular and infection microbiology · 2025Article
- Point-of-care test of blood Plasmodium RNA within a Pasteur pipette using a novel isothermal amplification without nucleic acid purification.Infectious diseases of poverty · 2024Article
- Lateral flow assay sensitivity and signal enhancement via laser µ-machined constrains in nitrocellulose membrane.Scientific reports · 2024Article
- Recombinase-aid amplification combined with lateral flow detection assay for sex identification of the great white pelican (Pelecanus onocrotalus).Scientific reports · 2024Article
- Advances in Malaria Diagnostic Methods in Resource-Limited Settings: A Systematic Review.Tropical medicine and infectious disease · 2024Review
- Development of Enzymatic Recombinase Amplification Assays for the Rapid Visual Detection of HPV16/18.Journal of microbiology and biotechnology · 2023Article
- Advances in the application of recombinase-aided amplification combined with CRISPR-Cas technology in quick detection of pathogenic microbes.Frontiers in bioengineering and biotechnology · 2023Review
- Rapid detection of multiple resistance genes to last-resort antibiotics inFrontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Malaria is a global public health problem. China has had no case of indigenous malaria since 2016. However, imported cases of malaria remain an issue among travelers, overseas workers, and foreign traders. Although these cases are always asymptomatic, if they donate blood, there is a great risk of transfusion transmitted-malaria (TTM). Therefore, blood banks need a rapid screening tool to detect Methods: We designed an assay using recombinase-aided amplification (RAA) and a lateral-flow dipstick (LFD) (RAA-LFD) to detect the 18S ribosomal RNA gene of Results: The RAA-LFD assay was performed in an incubator block at 37°C for 15 min, and the amplicons were visible to the naked eye on the flow dipsticks within 3 min. The sensitivity was 1 copy/μL of recombinant plasmid. For genomic DNA from whole blood of malaria patients infected with Conclusions: The results demonstrated that this RAA-LFD assay was suitable for reliable field detection of
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