Evidence map›Paper›PMID 35811616›Full record

ArticleJournal of pharmaceutical analysis2022

A preparation strategy for protein-oriented immobilized silica magnetic beads with Spy chemistry for ligand fishing.

Yu Yi, Jianming Hu, Shenwei Ding, Jianfeng Mei, Xudong Wang, Yanlu Zhang, Jianshu Chen, Guoqing Ying

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of pharmaceutical analysis, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yu YiInstitute of Biopharmaceuticals, College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310014, China.
Jianming HuInstitute of Biopharmaceuticals, College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310014, China.
Shenwei DingInstitute of Biopharmaceuticals, College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310014, China.
Jianfeng MeiInstitute of Biopharmaceuticals, College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310014, China.
Xudong WangInstitute of Biopharmaceuticals, College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310014, China.
Yanlu ZhangInstitute of Biopharmaceuticals, College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310014, China.
Jianshu ChenInstitute of Biopharmaceuticals, College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310014, China.
Guoqing YingInstitute of Biopharmaceuticals, College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310014, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Due to the complexity of bioactive ingredients in biological samples, the screening of target proteins is a complex process. Herein, a feasible strategy for directing protein immobilization on silica magnetic beads for ligand fishing based on SpyTag/SpyCatcher (ST/SC)-mediated anchoring is presented. Carboxyl functional groups on the surface of silica-coated magnetic beads (SMBs) were coupled with SC using the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride/

Indexed as

Drug screeningEDC/Sulfo-NHSGreen fluorescent proteinOriented immobilizationSilica magnetic beadsSpyTag/SpyCatcher

Identifiers

PMID35811616
PMCPMC9257657

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.