Evidence map›Paper›PMID 35811015›Full record

ArticleToxicology in vitro : an international journal published in association with BIBRA2022

The trichloroethylene metabolite S-(1,2-dichlorovinyl)-l-cysteine inhibits lipopolysaccharide-induced inflammation transcriptomic pathways and cytokine secretion in a macrophage cell model.

Sean M Harris, Kelly M Bakulski, John Dou, Ethan Houskamp, Eleanor C Scheeres, Emily Schellenboom, Olivia Harlow, Rita Loch-Caruso, Erica Boldenow

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Toxicology in vitro : an international journal published in association with BIBRA, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Sean M HarrisDepartment of Environmental Health Sciences, School of Public Health, University of Michigan, Ann Arbor, MI 48109-2029, USA. Electronic address: harrsemi@umich.edu.
Kelly M BakulskiDepartment of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, MI 48109-2029, USA. Electronic address: bakulski@umich.edu.
John DouDepartment of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, MI 48109-2029, USA. Electronic address: johndou@umich.edu.
Ethan HouskampDepartment of Biology, Calvin University, Grand Rapids, MI 49546-4402, USA. Electronic address: ejh64@students.calvin.edu.
Eleanor C ScheeresDepartment of Biology, Calvin University, Grand Rapids, MI 49546-4402, USA. Electronic address: ecs33@students.calvin.edu.
Emily SchellenboomDepartment of Biology, Calvin University, Grand Rapids, MI 49546-4402, USA. Electronic address: egs6@students.calvin.edu.
Olivia HarlowDepartment of Biology, Calvin University, Grand Rapids, MI 49546-4402, USA; Department of Immunology, Mayo Clinic, Rochester, MN 55905, USA.
Rita Loch-CarusoDepartment of Environmental Health Sciences, School of Public Health, University of Michigan, Ann Arbor, MI 48109-2029, USA. Electronic address: rlc@umich.edu.
Erica BoldenowDepartment of Biology, Calvin University, Grand Rapids, MI 49546-4402, USA. Electronic address: ejb25@calvin.edu.
Calvin University · USUniversity of Michigan–Ann Arbor · US

Funding

Michigan Institute for Clinical and Health Research (MICHR)UL1TR002240 · NCATS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI LUMENG, JULIE C, MASHOUR, GEORGE ALEXANDER · 2017 to 2022
$54.9M
Research Experiences and Training Coordination CoreP42ES017198 · NIEHS · NORTHEASTERN UNIVERSITY · PI HARRIS, SEAN M · 2010 to 2024
$38.6M
Strategic Vision & Impact on Environmental HealthP30ES017885 · NIEHS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Dana Dolinoy · 2011 to 2026
$21.3M
DNA Methylation, Genetics, and Modifiable Risk Factors of Dementia in a Nationally Representative, Multi-Ethnic Cohort - Diversity SupplementR01AG067592 · NIA · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI BAKULSKI, KELLY, WARE, ERIN BAKSHIS · 2020 to 2025
$3.8M
Racial inequalities in health throughout adulthood: The cumulative impact of neighborhood chemical and non-chemical stressors on epigenomic pathwaysR01MD013299 · NIMHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HICKEN, MARGARET TAKAKO · 2018 to 2022
$3.7M
Prenatal exposure to metals and risk for Autism Spectrum Disorder in MARBLES and EARLIR01ES025531 · NIEHS · JOHNS HOPKINS UNIVERSITY · PI VOLK, HEATHER E · 2016 to 2020
$3.2M
Folic Acid Prevention Pathways for ASD in High Risk FamiliesR01ES025574 · NIEHS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI SCHMIDT, REBECCA JEAN · 2015 to 2019
$3.1M
Characterizing disparities in late-onset Alzheimer's disease risk through polygenic risk and epidemiologic factors in the Health and Retirement SurveyR01AG055406 · NIA · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI BAKULSKI, KELLY, WARE, ERIN BAKSHIS · 2017 to 2020
$1.7M
NCATS NIH HHS UL1 TR002240NIA NIH HHS R01 AG055406NIA NIH HHS R01 AG067592NIEHS NIH HHS P30 ES017885NIEHS NIH HHS P42 ES017198NIEHS NIH HHS R01 ES025531NIEHS NIH HHS R01 ES025574NIMHD NIH HHS R01 MD013299
6 · The paper itself

Abstract

Studies have shown that the trichloroethylene metabolite S-(1,2-dichlorovinyl)-l-cysteine (DCVC) inhibits cytokine secretion in pathogen stimulated fetal membrane tissue but little is known about the mechanism for these effects, including which cell types or transcriptomic pathways are impacted. Macrophages play a critical role in fetal membrane immune responses during infection. We tested the hypothesis that DCVC inhibits lipopolysaccharide (LPS) stimulated inflammation pathways in macrophage-like THP-1 cells. We treated THP-1 cells for 24 h then treated with 1, 5, or 10 μM DCVC for 24 h. After a 4 h incubation with lipopolysaccharide (LPS), we collected RNA and cell media. We performed transcriptomic analysis using RNA sequencing for 5 μM DCVC treatments and quantified cytokine release (IL-1β, IL-6, and TNF-α) for 1, 5 and 10 μM DCVC treatments. RNA sequencing analysis revealed 1399 differentially expressed genes (FDR < 0.05 and log

Indexed as

CysteineTrichloroethyleneHumansInflammationInterleukin-6LipopolysaccharidesMacrophagesTranscriptomeTumor Necrosis Factor-alphaCysteineInterleukin-6LipopolysaccharidesTrichloroethyleneTumor Necrosis Factor-alphaCytokineInflammationMacrophagesToxicant-pathogen interactions

Identifiers

PMID35811015
PMCPMC10353343
OpenAlexW4284976681

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.