ReviewJournal of microscopy2022
How innovations in methodology offer new prospects for volume electron microscopy.
Review in Journal of microscopy, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- Classifying biophysical subpopulations of insulin secretory granules using quantitative whole-cell structure analysis.Structure (London, England : 1993) · 2026Article
- vEMRec: High-Resolution Volume Electron Microscopy Reconstruction Based on Structure-Preserving and High-Fidelity 3D Alignment.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- The Freiburg framework for multimodalFrontiers in synaptic neuroscience · 2026Article
- A workflow integrating organ-on-chip culture and correlative 3D light and electron microscopy for microtissue analysis.Scientific reports · 2025Article
- EMito-Metrix enables automated evaluation of mitochondrial morphology across species.Nature metabolism · 2025Article
- Classifying Biophysical Subpopulations of Insulin Secretory Granules using Quantitative Whole Cell Structure Analysis.bioRxiv : the preprint server for biology · 2025Article
- Reduction of SEM charging artefacts in native cryogenic biological samples.Nature communications · 2025Article
- Multiple sclerosis: what have we learned and can we still learn from electron microscopy.Cellular and molecular life sciences : CMLS · 2025Review
- Efficient cell-wide mapping of mitochondria in electron microscopic volumes using webKnossos.Cell reports methods · 2025Article
- Automated analysis of ultrastructure through large-scale hyperspectral electron microscopy.Npj imaging · 2024Article
- Visualizing Volumetric and Segmentation Data using Mol* Volumes & Segmentations 2.0.Current protocols · 2024Article
- Tracing nerve fibers with volume electron microscopy to quantitatively analyze brain connectivity.Communications biology · 2024Article
- A century journey of organelles research in the plant endomembrane system.The Plant cell · 2024Review
- Modular segmentation, spatial analysis and visualization of volume electron microscopy datasets.Nature protocols · 2024Review
- FAST-EM array tomography: a workflow for multibeam volume electron microscopy.Methods in microscopy · 2024Article
- The advent of preventive high-resolution structural histopathology by artificial-intelligence-powered cryogenic electron tomography.Frontiers in molecular biosciences · 2024Review
- Serial Block Face-Scanning Electron Microscopy as a Burgeoning Technology.Advanced biology · 2023Review
- Mol* Volumes and Segmentations: visualization and interpretation of cell imaging data alongside macromolecular structure data and biological annotations.Nucleic acids research · 2023Article
- How innovations in methodology offer new prospects for volume electron microscopy.Journal of microscopy · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Detailed knowledge of biological structure has been key in understanding biology at several levels of organisation, from organs to cells and proteins. Volume electron microscopy (volume EM) provides high resolution 3D structural information about tissues on the nanometre scale. However, the throughput rate of conventional electron microscopes has limited the volume size and number of samples that can be imaged. Recent improvements in methodology are currently driving a revolution in volume EM, making possible the structural imaging of whole organs and small organisms. In turn, these recent developments in image acquisition have created or stressed bottlenecks in other parts of the pipeline, like sample preparation, image analysis and data management. While the progress in image analysis is stunning due to the advent of automatic segmentation and server-based annotation tools, several challenges remain. Here we discuss recent trends in volume EM, emerging methods for increasing throughput and implications for sample preparation, image analysis and data management.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.