ArticleHistochemistry and cell biology2022
A technique for preparing undecalcified osteochondral fresh frozen sections for elemental mapping and understanding disease etiology.
Article in Histochemistry and cell biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- A novel tape-free sample preparation method for human osteochondral cryosections for high throughput hyperspectral imaging.Histochemistry and cell biology · 2024Article
- Spatial analysis of the osteoarthritis microenvironment: techniques, insights, and applications.Bone research · 2024Review
- Spatial distribution of elements during osteoarthritis disease progression using synchrotron X-ray fluorescence microscopy.Scientific reports · 2023Article
- Functional mass spectrometry imaging maps phospholipase-A2 enzyme activity during osteoarthritis progression.Theranostics · 2023Article
- In focus in HCB.Histochemistry and cell biology · 2022Article
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Authors and funding
6 authors.
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Abstract
The anatomy of the osteochondral junction is complex because several tissue components exist as a unit, including uncalcified cartilage (with superficial, middle, and deep layers), calcified cartilage, and subchondral bone. Furthermore, it is difficult to study because this region is made up of a variety of cell types and extracellular matrix compositions. Using X-ray fluorescence microscopy, we present a protocol for simultaneous elemental detection on fresh frozen samples. We transferred the osteochondral sample using a tape-assisted system and successfully tested it in synchrotron X-ray fluorescence. This protocol elucidates the distinct distribution of elements at the human knee's osteochondral junction, making it a useful tool for analyzing the co-distribution of various elements in both healthy and diseased states.
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