Evidence map›Paper›PMID 35804213›Full record

ArticleMolecular biology reports2022

Determination of genotoxic damages of picloram and dicamba with comet assay in Allium cepa rooted in tissue culture and distilled water.

Cigdem Alev Ozel, Fatma Unal, Ece Avuloglu-Yilmaz, Esra Erikel, Semra Mirici, Deniz Yuzbasioglu

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Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 16 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Cigdem Alev OzelFaculty of Gazi Education, Department of Biology Education, Gazi University, Ankara, Turkey. cigdemozel@gazi.edu.tr.ORCID http://orcid.org/0000-0002-5952-1412
Fatma UnalScience Faculty, Department of Biology, Gazi University, Ankara, Turkey.ORCID http://orcid.org/0000-0002-7468-6186
Ece Avuloglu-YilmazVocational School of Technical Sciences, Amasya University, Amasya, Turkey.ORCID http://orcid.org/0000-0002-5164-3431
Esra ErikelScience Faculty, Department of Biology, Gazi University, Ankara, Turkey.ORCID http://orcid.org/0000-0002-2058-2525
Semra MiriciFaculty of Gazi Education, Department of Biology Education, Gazi University, Ankara, Turkey.ORCID http://orcid.org/0000-0003-4999-8628
Deniz YuzbasiogluScience Faculty, Department of Biology, Gazi University, Ankara, Turkey.ORCID http://orcid.org/0000-0003-2756-7712
Gazi University · TRAmasya Üniversitesi · TR

Funding

Gazi Üniversitesi Gazi Üniversitesi
6 · The paper itself

Abstract

backgroundMany genotoxicity tests allow us to understand the mechanism of damages on genetic material occurring in living organisms against various physical and chemical agents. One of them is the Comet test. The current study aimed to evaluate genotoxic caused by picloram and dicamba to root meristems of Allium cepa utilizing comet assay.

methodsTwo different protocols were used for rooting and auxin/pesticide application. (i) A. cepa bulbs were rooted in MS medium and then treated with Murashige and Skoog (MS) medium (control) and 0.67, 1.34, 2.01, 2.68, 3.35, 4.02, and 8.04 mg/L of picloram and dicamba using aseptic tissue culture techniques. (ii) A. cepa bulbs were then rooted in bidistilled water and treated with 0 (control), 0.67, 1.34, 2.01, 2.68, 3.35, 4.02, and 8.04 mg/L of picloram and dicamba in distilled water. The A. cepa root tip cells in both treatment groups were examined using comet test to find the possible DNA damaging effects of picloram and dicamba.

resultsThe results obtained at all the concentrations were statistically compared with their control groups. Almost at all the concentrations of Picloram and dicamba increased comet tail intensity (%) and tail moment in roots treated in MS medium. Two highest concentrations revealed toxic effect. On the other hand, DNA damaging effect of both auxins was only noted on the highest (> 4.02 mg/L) in roots treated in distilled water.

conclusionsThis study approve and confirm genotoxic effects of how growth regulators on plants. These findings give an evidence of DNA damage in A. cepa. Therefore, both picloram and dicamba should only be used in appropriate and recommended concentrations in agriculture to conserve ecosystem and to pose minimum threat to life.

Indexed as

DicambaOnionsChromosome AberrationsComet AssayDNA DamageEcosystemPicloramWaterDicambaPicloramWaterAllium cepaComet AssayDicambaDNADNA damageGenotoxicityPicloram

Identifiers

PMID35804213
OpenAlexW4284970711

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.