ArticleScientific reports2022
Rapid reverse genetics systems for Nothobranchius furzeri, a suitable model organism to study vertebrate aging.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 17 citations in OpenAlex.
- Long read sequencing of retinal RNA improves killifish transcriptome annotation.bioRxiv : the preprint server for biology · 2026Article
- Identification of gradual aging and late-onset aging markers using male African turquoise killifish.Scientific reports · 2026Article
- ER Stress Ire1-Xbp1s Pathway Maintains Youthful Epidermal Basal Layer Through the Regulation of Cell Proliferation.Aging cell · 2025Article
- A short-lived fish with long-lasting effects: hallmarks of aging inFrontiers in aging · 2025Review
- Mechano-gradients drive morphogen-noise correction to ensure robust patterning.Science advances · 2024Article
- The dwarf neon rainbowfish Melanotaenia praecox, a small spiny-rayed fish with potential as a new Acanthomorpha model fish: II. Establishment of a microinjection procedure for genetic engineering.Developmental dynamics : an official publication of the American Association of Anatomists · 2024Article
- The African Turquoise Killifish: A Scalable Vertebrate Model for Aging and Other Complex Phenotypes.Cold Spring Harbor protocols · 2024Article
- Widespread sex dimorphism across single-cell transcriptomes of adult African turquoise killifish tissues.Cell reports · 2023Article
- Transcriptomes of aging brain, heart, muscle, and spleen from female and male African turquoise killifish.Scientific data · 2023Article
- Article
- Generation of a transparent killifish line through multiplex CRISPR/Cas9mediated gene inactivation.eLife · 2023Article
- Age-Related Alterations in the Level and Metabolism of Serotonin in the Brain of Males and Females of Annual Turquoise Killifish (International journal of molecular sciences · 2023Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The African turquoise killifish Nothobranchius furzeri (N. furzeri) is a useful model organism for studying aging, age-related diseases, and embryonic diapause. CRISPR/Cas9-mediated gene knockout and Tol2 transposon-mediated transgenesis in N. furzeri have been reported previously. However, these methods take time to generate knockout and transgenic fish. In addition, knock-in technology that inserts large DNA fragments as fluorescent reporter constructs into the target gene in N. furzeri has not yet been established. Here, we show that triple-target CRISPR-mediated single gene disruption efficiently produces whole-body biallelic knockout and enables the examination of gene function in the F0 generation. In addition, we developed a method for creating the knock-in reporter N. furzeri without crossing by optimizing the CRISPR/Cas9 system. These methods drastically reduce the duration of experiments, and we think that these advances will accelerate aging and developmental studies using N. furzeri.
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