ArticleNature communications2022
A luciferase prosubstrate and a red bioluminescent calcium indicator for imaging neuronal activity in mice.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- Longitudinal in vivo imaging reveals asynchronous, incomplete Nipah virus clearance with prolonged focal CNS involvement in IFNAREmerging microbes & infections · 2026Article
- Labeling and imaging of neurons with a genetically encoded autonomous bioluminescence system.Scientific reports · 2026Article
- Ablation of prostaglandin ENature biomedical engineering · 2026Article
- Protein engineering: status report.Protein engineering, design & selection : PEDS · 2026Review
- An Enhanced Red Bioluminescent Indicator for Responsive Detection of Physiological Calcium Dynamics in Cells and Mice.ACS sensors · 2025Article
- An optimized luciferin formulation for NanoLuc-based in vivo bioluminescence imaging.Scientific reports · 2025Article
- Coelenterazine Analogs for Bioassays and Molecular Imaging.Sensors (Basel, Switzerland) · 2025Review
- PEGylated ATP-Independent Luciferins for Noninvasive High-Sensitivity High-Speed Bioluminescence Imaging.ACS chemical biology · 2025Article
- Quantitative approaches for studying G protein-coupled receptor signalling and pharmacology.Journal of cell science · 2025Review
- Calcium Indicators with Fluorescence Lifetime-Based Signal Readout: A Structure-Function Study.International journal of molecular sciences · 2024Article
- Optimization of Bangladesh and Malaysian genotype recombinant reporter Nipah viruses for in vitro antiviral screening and in vivo disease modeling.Antiviral research · 2024Article
- Imaging bioluminescence by detecting localized haemodynamic contrast from photosensitized vasculature.Nature biomedical engineering · 2024Article
- Bright Red Bioluminescence from Semisynthetic NanoLuc (sNLuc).ACS chemical biology · 2024Article
- Bioluminescence Imaging of Potassium Ion Using a Sensory Luciferin and an Engineered Luciferase.Journal of the American Chemical Society · 2024Article
- Fluorescent Protein-Based Sensors for Detecting Essential Metal Ions across the Tree of Life.ACS sensors · 2024Review
- Toward a brighter constellation: multiorgan neuroimaging of neural and vascular dynamics in the spinal cord and brain.Neurophotonics · 2024Article
- Bioluminescence as a functional tool for visualizing and controlling neuronal activityNeurophotonics · 2024Article
- Method for optimizing imaging parameters to record neuronal and cellular activity at depth with bioluminescence.Neurophotonics · 2024Article
- Recent advances in bioluminescent probes for neurobiology.Neurophotonics · 2024Article
- Current advances in the development of bioluminescent probes toward spatiotemporal trans-scale imaging.Biophysics and physicobiology · 2024Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Although fluorescent indicators have been broadly utilized for monitoring bioactivities, fluorescence imaging, when applied to mammals, is limited to superficial targets or requires invasive surgical procedures. Thus, there is emerging interest in developing bioluminescent indicators for noninvasive mammalian imaging. Bioluminescence imaging (BLI) of neuronal activity is highly desired but hindered by insufficient photons needed to digitalize fast brain activities. In this work, we develop a luciferase prosubstrate deliverable at an increased dose and activated in vivo by nonspecific esterase. We further engineer a bright, bioluminescent indicator with robust responsiveness to calcium ions (Ca
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.