Evidence map›Paper›PMID 35803124›Full record

ArticleRedox biology2022

Measuring peroxidasin activity in live cells using bromide addition for signal amplification.

Veronika F S Pape, Hajnal A Kovács, István Szatmári, Imre Ugrai, Bence Szikora, Imre Kacskovics, Zoltán May, Norbert Szoboszlai, Gábor Sirokmány, Miklós Geiszt

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Redox biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. The role of peroxidasin in solid cancer progression.Biochemical Society transactions · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 1 country.

Veronika F S PapeDepartment of Physiology, Semmelweis University, Faculty of Medicine, Tűzoltó utca 37-47, H-1094, Budapest, Hungary. Electronic address: veronika.pape@med.semmelweis-univ.hu.
Hajnal A KovácsDepartment of Physiology, Semmelweis University, Faculty of Medicine, Tűzoltó utca 37-47, H-1094, Budapest, Hungary.
István SzatmáriInstitute of Pharmaceutical Chemistry and Stereochemistry Research Group of Hungarian Academy of Sciences, University of Szeged, Eötvös u. 6, H-6720, Szeged, Hungary.
Imre UgraiInstitute of Pharmaceutical Chemistry and Stereochemistry Research Group of Hungarian Academy of Sciences, University of Szeged, Eötvös u. 6, H-6720, Szeged, Hungary.
Bence SzikoraImmunoGenes Ltd., Budakeszi, Hungary.
Imre KacskovicsImmunoGenes Ltd., Budakeszi, Hungary.
Zoltán MayResearch Centre for Natural Sciences, Eötvös Loránd Research Network, Magyar Tudósok körútja 2, H-1117, Budapest, Hungary.
Norbert SzoboszlaiInstitute of Chemistry, Eötvös Loránd University, Pázmány Péter sétány 1/A, H-1117, Budapest, Hungary.
Gábor SirokmányDepartment of Physiology, Semmelweis University, Faculty of Medicine, Tűzoltó utca 37-47, H-1094, Budapest, Hungary.
Miklós GeisztDepartment of Physiology, Semmelweis University, Faculty of Medicine, Tűzoltó utca 37-47, H-1094, Budapest, Hungary. Electronic address: geiszt.miklos@med.semmelweis-univ.hu.
Semmelweis University · HUImmunoGenes (Hungary) · HUUniversity of Szeged · HUEötvös Loránd University · HUHungarian Research Network · HU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Peroxidasin (PXDN) is involved in the crosslinking of collagen IV, a major constituent of basement membranes. Disruption of basement membrane integrity as observed in genetic alterations of collagen IV or PXDN can result in developmental defects and diverse pathologies. Hence, the study of PXDN activity in (patho)physiological contexts is highly relevant. So far, measurements of PXDN activity have been reported from purified proteins, cell lysates and de-cellularized extracellular matrix. Here, for the first time we report the measurement of PXDN activity in live cells using the Amplex Red assay with a signal amplifying modification. We observe that bromide addition enhances the obtained signal, most likely due to formation of HOBr. Abrogation of signal amplification by the HOBr scavenger carnosine supports this hypothesis. Both, pharmacological inhibition as well as complementary genetic approaches confirm that the obtained signal is indeed related to PXDN activity. We validate the modified assay by investigating the effect of Brefeldin A, to inhibit the secretory pathway and thus the access of PXDN to the extracellular Amplex Red dye. Our method opens up new possibilities to investigate the activity of PXDN in (patho)physiological contexts.

Indexed as

BromidesExtracellular Matrix ProteinsCollagen Type IVPeroxidasePeroxidasinBromidesCollagen Type IVExtracellular Matrix ProteinsPeroxidasePeroxidasinAmplex redBrefeldin ACarnosineHypobromous acidPeroxidasin

Identifiers

PMID35803124
PMCPMC9287737
OpenAlexW4283729714

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.