ArticlePloS one2022
Direct addition of poly-lysine or poly-ethylenimine to the medium: A simple alternative to plate pre-coating.
Article in PloS one, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Xeno-free production of lentiviral particles: implementing hPL as FBS replacement in cell culture.Frontiers in toxicology · 2026Article
- Isolation and Culture of Neonatal Mouse Atrial and Ventricular Myocytes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Qualification of a quantitative stability indicating potency assay for mRNA-LNP vaccine candidates.Gates open research · 2026Article
- Effects of Chronic Elevation in Plasma Membrane Cholesterol on the Function of Human NaLivers · 2025Article
- Ultrathin ALD Coatings of Zr and V Oxides on Anodic TiOACS applied materials & interfaces · 2025Article
- Enhancing lentiviral production for WAS gene therapy: a comparative analysis of stable producer cell lines evaluating flatware system and adherent bioreactors in perfusion mode.Frontiers in bioengineering and biotechnology · 2025Article
- Enhancing biocompatibility of the brain-machine interface: A review.Bioactive materials · 2024Review
- Development of a highly sensitive platform for protein-protein interaction detection and regulation of T cell function.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Biomaterial strategies for regulating the neuroinflammatory response.Materials advances · 2024Review
- Mimicking the Physicochemical Properties of the Cornea: A Low-Cost Approximation Using Highly Available Biopolymers.Polymers · 2024Article
- 3D topographies promote macrophage M2d-Subset differentiation.Materials today. Bio · 2024Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For most cell culture experiments, it is indispensable that the cells are firmly anchored to culture plates, withstanding rinsing steps that can create shear forces and tolerating temperature changes without detaching. For semi-adherent cells such as the common HEK 293 or PC-12 cells, this could so far be obtained by time-consuming plate pre-coating with cationic polymer solutions. We report here, that i) pre-coating with the cheaper poly-ethylenimine (PEI) works as well as the commonly used poly-D-lysine (PDL), but more importantly and novel ii) that simple direct addition of either PEI (1.5 μg/ml) or PDL (2 μg/ml) to the cell culture medium results in strongly anchored HEK 293 cells, indistinguishable from ones seeded on pre-coated plates. Therefore, the replacement of plate pre-coating by direct addition of either PEI or PDL gives comparable excellent results, but is highly labour-, time-, and cost-efficient. Moreover, we could show that addition of PDL or PEI also works similarly well in animal-free culture using human platelet lysate instead of fetal bovine serum. Interestingly, additional experiments showed that strong cell attachment requires only cationic polymers but not fetal bovine serum or human platelet lysate added to the medium.
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