ArticleNucleic acids research2022
The molecular mechanism of microRNA duplex selectivity of Arabidopsis ARGONAUTE10.
Article in Nucleic acids research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it, 22 citations in OpenAlex.
- The role of microRNAs in responses to drought and heat stress in peanut (Arachis hypogaea).The plant genome · 2023Pooled it
- Decoding Argonaute specificity: insights fromRNA (New York, N.Y.) · 2026Review
- Plant microRNA maturation and function.Nature reviews. Molecular cell biology · 2026Review
- Principles of miRNA/miRNA* function in plant MIRNA processing.Nucleic acids research · 2024Article
- Interaction between a J-domain co-chaperone and a specific Argonaute protein contributes to microRNA function in animals.Nucleic acids research · 2024Article
- An AGO10:miR165/6 module regulates meristem activity and xylem development in the Arabidopsis root.The EMBO journal · 2024Article
- Genome-wide identification and in silico characterization of major RNAi gene families in date palm (Phoenix dactylifera).BMC genomic data · 2024Article
- The plant siRNA landscape.The Plant cell · 2024Review
- Beyond Loading: Functions of Plant ARGONAUTE Proteins.International journal of molecular sciences · 2023Review
- A tiny loop in the Argonaute PIWI domain tunes small RNA seed strength.EMBO reports · 2023Article
- The mechanisms of siRNA selection by plant Argonaute proteins triggering DNA methylation.Nucleic acids research · 2022Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
Small RNAs (sRNAs), including microRNAs (miRNAs) and small interfering RNAs (siRNAs), are essential gene regulators for plant and animal development. The loading of sRNA duplexes into the proper ARGONAUTE (AGO) protein is a key step to forming a functional silencing complex. In Arabidopsis thaliana, the specific loading of miR166/165 into AGO10 (AtAGO10) is critical for the maintenance of the shoot apical meristem, the source of all shoot organs, but the mechanism by which AtAGO10 distinguishes miR166/165 from other cellular miRNAs is not known. Here, we show purified AtAGO10 alone lacks loading selectivity towards miR166/165 duplexes. However, phosphate and HSP chaperone systems reshape the selectivity of AtAGO10 to its physiological substrates. A loop in the AtAGO10 central cleft is essential for recognizing specific mismatches opposite the guide strand 3' region in miR166/165 duplexes. Replacing this loop with the equivalent loop from Homo sapiens AGO2 (HsAGO2) changes AtAGO10 miRNA loading behavior such that 3' region mismatches are ignored and mismatches opposite the guide 5' end instead drive loading, as in HsAGO2. Thus, this study uncovers the molecular mechanism underlying the miR166/165 selectivity of AtAGO10, essential for plant development, and provides new insights into how miRNA duplex structures are recognized for sRNA sorting.
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