Evidence map›Paper›PMID 35799906›Full record

ArticleBio-protocol2022

Protocol to Isolate Germinal Centers by Laser Microdissection.

Farbod Bahreini, Markus Niebuhr, Julia Belde, Jürgen Westermann, Kathrin Kalies

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 54% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Farbod BahreiniInstitute of Anatomy, University of Luebeck, Luebeck, Germany.
Markus NiebuhrInstitute of Anatomy, University of Luebeck, Luebeck, Germany.
Julia BeldeInstitute of Anatomy, University of Luebeck, Luebeck, Germany.
Jürgen WestermannInstitute of Anatomy, University of Luebeck, Luebeck, Germany.
Kathrin KaliesInstitute of Anatomy, University of Luebeck, Luebeck, Germany.
University of Lübeck · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

During adaptive immune responses, germinal centers (GC) appear as transient microstructures, in which antigen-specific B and T cells interact with each other. Because only the antigen-activated B and T cells, such as Plasmablasts or follicular T helper (Tfh) cells, are present in GC, the in depth-analysis of GC is of great interest. To identify the cells that reside within GC, the majority of studies use the expression of specific surface molecules for analysis by flow cytometry. To do so, the tissue has to be disrupted for the preparation of single-cell suspensions. Thereby, the local information regarding neighborhoods of B cells and T cells and their potential interaction is lost. To study GC

Indexed as

Cryo-preserved lymphoid structuresFollicular T helper cellsGerminal centersIn vivo analysisLaser microdissectionSecondary lymphoid organs

Identifiers

PMID35799906
PMCPMC9243518
OpenAlexW4284964144

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.