Evidence map›Paper›PMID 35799320›Full record

ArticleThe Journal of small animal practice2023

Urinary extracellular vesicles as a source of protein-based biomarkers in feline chronic kidney disease and hypertension.

J S Lawson, H M Syme, P R Antrobus, J M Karttunen, S E Stewart, F E Karet Frankl, T L Williams

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of small animal practice, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 3 countries.

J S LawsonClinical Sciences and Services, The Royal Veterinary College, Hatfield, AL9 7TA, UK.
H M SymeCambridge Institute for Medical Research, University of Cambridge, Keith Peters Building, Cambridge, CB2 0XY, UK.ORCID 0000-0002-0967-4031
P R AntrobusCambridge Institute for Medical Research, University of Cambridge, Keith Peters Building, Cambridge, CB2 0XY, UK.
J M KarttunenDepartment of Veterinary Medicine, The Queen's Veterinary School Hospital, University of Cambridge, Cambridge, CB3 0ES, UK.
S E StewartDepartment of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Victoria, 3086, Australia.
F E Karet FranklCambridge Institute for Medical Research, University of Cambridge, Keith Peters Building, Cambridge, CB2 0XY, UK.
T L WilliamsDepartment of Veterinary Medicine, The Queen's Veterinary School Hospital, University of Cambridge, Cambridge, CB3 0ES, UK.
University of Cambridge · GBLa Trobe University · AURoyal Veterinary College · GB

Funding

PetSavers Clinical Research Grant 10.19
6 · The paper itself

Abstract

objectivesTo validate a methodology for isolating feline urinary extracellular vesicles and characterise the urinary extracellular vesicle population and proteome in cats with normal renal function and cats with normotensive or hypertensive chronic kidney disease.

methodsFeline urinary extracellular vesicles were isolated using three different methods (precipitation alone, precipitation followed by size exclusion chromatography and ultrafiltration followed by size exclusion chromatography, which were compared via transmission electron microscopy and nanoparticle tracking analysis. Cats with normal renal function (n=9), normotensive chronic kidney disease (n=10) and hypertensive chronic kidney disease (n=9) were identified and urinary extracellular vesicles isolated from patient urine samples via ultrafiltration followed by size exclusion chromatography. Extracellular vesicle size and concentration were determined using nanoparticle tracking analysis, and subsequently underwent proteomic analysis using liquid chromatography with tandem mass spectrometry to identify differences in protein expression between categories.

resultsUrinary extracellular vesicle preparations contained particles of the expected size and morphology, and those obtained by ultrafiltration + size exclusion chromatography had a significantly higher purity (highest particle: protein ratio). The urinary extracellular vesicle proteomes contained extracellular vesicle markers and proteins originating from all nephron segments. Urinary extracellular vesicle concentration and size were unaffected by renal disease or hypertension. There were no differentially expressed proteins detected when comparing urinary extracellular vesicles derived from cats in the healthy category with the combined chronic kidney disease category, but five differentially expressed proteins were identified between the normotensive chronic kidney disease and hypertensive chronic kidney disease categories. CLINICAL SIGNIFICANCE: Feline urinary extracellular vesicles can be successfully isolated from stored urine samples. Differentially expressed urinary extracellular vesicle proteins were discovered in cats with hypertensive chronic kidney disease, and warrant further investigation into their utility as biomarkers or therapeutic targets.

Indexed as

Cat DiseasesExtracellular VesiclesHypertension, RenalRenal Insufficiency, ChronicAnimalsBiomarkersCatsNephritisProteomeProteomicsBiomarkersProteome

Identifiers

PMID35799320
PMCPMC10084206
OpenAlexW4284889223

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.