ArticleScientific reports2022
High rates of plasmid cotransformation in E. coli overturn the clonality myth and reveal colony development.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Mesolens imaging in microbiology.Essays in biochemistry · 2026Review
- Designing and Evaluation of a Novel IL-1RA Fusion Cytokine to Enhance the Pharmacokinetics and Receptor Affinity for Better Therapeutic Intervention in Inflammatory Disorders.Current computer-aided drug design · 2026Article
- Plasmid2MC: efficient cell-free generation of high-purity minicircle DNA for genome editing in mammalian cells.Communications biology · 2025Article
- How many plasmids can bacteria carry? A synthetic biology perspective.Open biology · 2025Review
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- Genomic analysis of antimicrobial resistant Escherichia coli isolated from manure and manured agricultural grasslands.npj antimicrobials and resistance · 2025Article
- Directed Evolution of Silicatein Reveals Biomineralization Synergism between Protein Sequences.ACS omega · 2025Article
- Real-time imaging of bacterial colony growth dynamics for cells with Type IV-A1 CRISPR-Cas activity.microLife · 2025Article
- Resistance mechanism of Escherichia coli strains with different ampicillin resistance levels.Applied microbiology and biotechnology · 2024Article
- Quantifying the fractal complexity of nutrient transport channels inMicrobiology (Reading, England) · 2024Article
- No two clones are alike: characterization of heterologous subpopulations in a transgenic cell line of the model diatom Phaeodactylum tricornutum.Microbial cell factories · 2024Article
- CRISPR/Cas-Mediated Knockdown of PD-L1 and KRAS in Lung Cancer Cells.International journal of molecular sciences · 2024Article
- Selection and characterization of human scFvs targeting the SARS-CoV-2 nucleocapsid protein isolated from antibody libraries of COVID-19 patients.Scientific reports · 2024Article
- ORBIT for E. coli: kilobase-scale oligonucleotide recombineering at high throughput and high efficiency.Nucleic acids research · 2024Article
- Efficient modification and preparation of circular DNA for expression in cell culture.Communications biology · 2022Article
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Authors and funding
4 authors.
Funding
Abstract
The concept of DNA transfer between bacteria was put forth by Griffith in 1928. During the dawn of molecular cloning of DNA in the 1980s, Hanahan described how the transformation of DNA plasmids into bacteria would allow for cloning of DNA fragments. Through this foundational work, it is widely taught that a typical transformation produces clonal bacterial colonies. Using low concentrations of several plasmids that encode different fluorescent proteins, under the same selective antibiotic, we show that E. coli bacteria readily accept multiple plasmids, resulting in widespread aclonality and reveal a complex pattern of colony development. Cotransformation of plasmids occurs by either CaCl
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