Evidence map›Paper›PMID 35797032›Full record

ArticleACS synthetic biology2022

FRAGLER: A Fragment Recycler Application Enabling Rapid and Scalable Modular DNA Assembly.

David Öling, Olivier Lan-Chow-Wing, Andrea Martella, Samuel Gilberto, Jordi Chi, Emily Cooper, Tora Edström, Bo Peng, Dean Sumner, Fredrik Karlsson and 3 more

Abstract read
In one paragraph

Article in ACS synthetic biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. The design and engineering of synthetic genomes.Nature reviews. Genetics · 2025
    Review
  4. Article
  5. GGAssembler: Precise and economical design and synthesis of combinatorial mutation libraries.Protein science : a publication of the Protein Society · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

David ÖlingDiscovery Sciences, Biopharmaceuticals R&D, AstraZeneca, 43183 Gothenburg, Sweden.ORCID 0000-0002-3390-3543
Olivier Lan-Chow-WingDiscovery Sciences, Biopharmaceuticals R&D, AstraZeneca, CB2 0AA Cambridge, U.K.
Andrea MartellaDiscovery Sciences, Biopharmaceuticals R&D, AstraZeneca, CB2 0AA Cambridge, U.K.ORCID 0000-0002-0376-0799
Samuel GilbertoDiscovery Sciences, Biopharmaceuticals R&D, AstraZeneca, CB2 0AA Cambridge, U.K.
Jordi ChiDiscovery Sciences, Biopharmaceuticals R&D, AstraZeneca, 43183 Gothenburg, Sweden.ORCID 0000-0002-6633-691X
Emily CooperDiscovery Sciences, Biopharmaceuticals R&D, AstraZeneca, CB2 0AA Cambridge, U.K.
Tora EdströmDiscovery Sciences, Biopharmaceuticals R&D, AstraZeneca, 43183 Gothenburg, Sweden.
Bo PengDiscovery Sciences, Biopharmaceuticals R&D, AstraZeneca, 43183 Gothenburg, Sweden.
Dean SumnerDiscovery Sciences, Biopharmaceuticals R&D, AstraZeneca, 43183 Gothenburg, Sweden.
Fredrik KarlssonDiscovery Sciences, Biopharmaceuticals R&D, AstraZeneca, 43183 Gothenburg, Sweden.
Petr VolkovDiscovery Sciences, Biopharmaceuticals R&D, AstraZeneca, 43183 Gothenburg, Sweden.
Carl I WebsterDiscovery Sciences, Biopharmaceuticals R&D, AstraZeneca, CB2 0AA Cambridge, U.K.
Robert RothDiscovery Sciences, Biopharmaceuticals R&D, AstraZeneca, 43183 Gothenburg, Sweden.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Rapid and flexible plasmid construct generation at scale is one of the most limiting first steps in drug discovery projects. These hurdles can partly be overcome by adopting modular DNA design principles, automated sequence fragmentation, and plasmid assembly. To this end we have designed a robust, multimodule

Indexed as

COVID-19Escherichia coliCloning, MolecularDNAGenetic VectorsHumansPlasmidsRNA, ViralSARS-CoV-2Synthetic BiologyDNARNA, Viral

Identifiers

PMID35797032
PMCPMC9295698

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.