ArticleMethods in molecular biology (Clifton, N.J.)2022
Cloning, Sequencing, and Linkage Analysis of piRNAs.
Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 5 citations in OpenAlex.
- Article
- Retrovirus insertions in host transcripts trigger de novo piRNA immunity.The EMBO journal · 2026Article
- miRNA/siRNA-directed pathway to produce noncoding piRNAs from endogenous protein-coding regions ensuresScience advances · 2023Article
- The absence of core piRNA biogenesis factors does not impact efficient transposon silencing in Drosophila.PLoS biology · 2023Article
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Authors and funding
1 author at 1 institution in 1 country.
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Abstract
Piwi-interacting RNAs (piRNAs) are 25- to 32-nucleotide-long small RNAs that silence transposable elements (transposons) in animal gonads. piRNAs have a large sequence diversity (over one million different sequences per organism) to target a variety of transposon sequences. This is achieved by flexible and distinct biogenesis pathways that are evolutionarily conserved. In this chapter, I describe a detailed method of purifying and cloning piRNAs from freshly dissected tissue samples, such as fruit fly ovaries, for the high-throughput sequencing. I also describe how to computationally process the sequencing data and interrogate the characteristic pattern of piRNA biogenesis, including ping-pong amplification and head-to-tail phasing.
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