ArticleDatabase : the journal of biological databases and curation2022
Curation of a reference database of COI sequences for insect identification through DNA metabarcoding: COins.
Article in Database : the journal of biological databases and curation, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed.
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- Decoding Biodiversity in Baiyangdian Lake: A DNA Barcode Reference Library for Aquatic Insects.Insects · 2026Article
- Evaluation of Oxford Nanopore Technologies MinION Sequencer as a Novel Short Amplicon Metabarcoding Tool Using Arthropod Mock Sample and Irish Bat Diet Characterisation.Ecology and evolution · 2025Article
- A novel taxonomic database for eukaryotic mitochondrial cytochrome oxidase subunit I gene (eKOI), with a focus on protists diversity.Database : the journal of biological databases and curation · 2025Article
- In-situ diet-microbiota associations across taxonomic scales in desert-dwelling amphibians and reptiles.ISME communications · 2025Article
- WiPFIM: A digital platform for interlinking biocollections of wild plants, fruits, associated insects, and their molecular barcodes.Ecology and evolution · 2024Article
- Filling knowledge gaps in insect conservation by leveraging genetic data from public archives.Database : the journal of biological databases and curation · 2024Article
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- Interrogating 1000 insect genomes for NUMTs: A risk assessment for estimates of species richness.PloS one · 2023Article
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- Taxonomic identification accuracy from BOLD and GenBank databases using over a thousand insect DNA barcodes from Colombia.PloS one · 2023Article
- Looking to the past to inform the future: What eDNA from herbarium specimens can tell us about plant-animal interactions.Applications in plant sciencesArticle
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7 authors.
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Abstract
DNA metabarcoding is a widespread approach for the molecular identification of organisms. While the associated wet-lab and data processing procedures are well established and highly efficient, the reference databases for taxonomic assignment can be implemented to improve the accuracy of identifications. Insects are among the organisms for which DNA-based identification is most commonly used; yet, a DNA-metabarcoding reference database specifically curated for their species identification using software requiring local databases is lacking. Here, we present COins, a database of 5' region cytochrome c oxidase subunit I sequences (COI-5P) of insects that includes over 532 000 representative sequences of >106 000 species specifically formatted for the QIIME2 software platform. Through a combination of automated and manually curated steps, we developed this database starting from all COI sequences available in the Barcode of Life Data System for insects, focusing on sequences that comply with several standards, including a species-level identification. COins was validated on previously published DNA-metabarcoding sequences data (bulk samples from Malaise traps) and its efficiency compared with other publicly available reference databases (not specific for insects). COins can allow an increase of up to 30% of species-level identifications and thus can represent a valuable resource for the taxonomic assignment of insects' DNA-metabarcoding data, especially when species-level identification is needed https://doi.org/10.6084/m9.figshare.19130465.v1.
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