ArticleThe Journal of clinical endocrinology and metabolism2022
Characterization of Aldosterone-producing Cell Cluster (APCC) at Single-cell Resolution.
Article in The Journal of clinical endocrinology and metabolism, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Inter- and intratumoral heterogeneity of aldosterone-producing adenomas: recent advances and clinical implications.Endocrine journal · 2026Review
- From bench to bedside: primary aldosteronism revisited.Endocrine connections · 2026Article
- Integrated Transcriptomics Reveals Evolutionary Trajectories and Cell Density-Dependent Mechanisms in Aldosterone-Producing Adenomas.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Single-nucleus RNA sequencing reveals the cellular composition and the mechanism underlying adrenal myelolipoma.Endocrine · 2026Article
- 'Glucocorticoids, Cushing's syndrome and cellular senescence: a mechanistic link to metabolic ageing'.Endocrine connections · 2026Review
- Mild autonomous cortisol secretion in primary aldosteronism: the origin of cortisol.Hypertension research : official journal of the Japanese Society of Hypertension · 2025Article
- Single-Cell Atlas Reveals Tumorigenic Profiles and Immune Dynamics of Adrenal Incidentalomas.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Development and validation of prediction models for special subtype of primary aldosteronism: patients with negative adrenal CT imaging.Frontiers in endocrinology · 2025Article
- Beyond pharmacotherapy: bilateral adrenal artery embolization as a potential game-changer for idiopathic hyperaldosteronism patients.Hypertension research : official journal of the Japanese Society of Hypertension · 2025Article
- Steroids-producing nodules: a two-layered adrenocortical nodular structure as a precursor lesion of cortisol-producing adenoma.EBioMedicine · 2024Article
- In Situ Spatial Reconstruction of Distinct Normal and Pathological Cell Populations Within the Human Adrenal Gland.Journal of the Endocrine Society · 2023Article
- Review
- Hormones and Aging: An Endocrine Society Scientific Statement.The Journal of clinical endocrinology and metabolism · 2023Article
- Imaging or Adrenal Vein Sampling Approach in Primary Aldosteronism? A Patient-Based Approach.Tomography (Ann Arbor, Mich.) · 2022Review
Corrections and comments
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Authors and funding
14 authors at 5 institutions in 2 countries.
Funding
Abstract
contextThe adrenal cortex consists of zona glomerulosa (ZG), fasciculata (ZF), and reticularis. Aldosterone-producing cell clusters (APCCs) that strongly express aldosterone synthase (CYP11B2) are frequently found in adult adrenals and harbor somatic mutations that are also detected in aldosterone-producing adenomas (APAs). Primary aldosteronism is mainly caused by APAs or idiopathic hyperaldosteronism (IHA). We presume that APCCs are causing IHA and are precursors of APAs. However, the gene expression characteristics and especially the development of APCCs are not well understood.
objectiveThis study aimed to analyze the transcriptome of APCCs at single-cell resolution and infer the developmental trajectory.
methodsSingle-cell RNA sequencing (scRNA-seq) of 2 adult adrenals was performed.
resultsImmunohistochemical analyses confirmed the 2 adrenals had APCCs. scRNA-seq data of 2928 adrenal cells were obtained and 1765 adrenocortical cells were identified based on unsupervised clustering and the marker gene expression. The adrenocortical cells were divided into 6 clusters, of which 3 clusters (923 cells) were composed of APCC/ZG cells. By further subclustering, the APCC/ZG cells were divided into 3 clusters (clusters C1, C2, and C3), we finally identified APCC cluster (C3) and ZG cluster (C1). Cluster C2 seemed to be ZG-to-ZF transitional cells. RNA velocity analysis inferred the developmental direction from cluster ZG-cluster-C1 to APCC-cluster-C3. The scRNA-seq additionally revealed that many CYP11B2-positive cells were positive for CYP11B1 and/or CYP17A1, which were essential for cortisol but not for aldosterone production.
conclusionsOur results revealed the gene expression characteristics of APCC at single-cell resolution and show that some ZG cells remodel to APCC.
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