ArticleNature communications2022
Transcriptional programming in a Bacteroides consortium.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
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- Living Microbial Drugs.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Review
- Engineering the Gut Microbiome: Emerging Genome-Editing Strategies and Therapeutic Applications.Microorganisms · 2026Review
- Protecting cells at the genetic level and simulating unauthorized access via a biohackathon.Science advances · 2026Article
- Polydopamine-chitosan coated biofilm-stateMaterials today. Bio · 2025Article
- Credible inferences in microbiome research: ensuring rigour, reproducibility and relevance in the era of AI.Nature reviews. Gastroenterology & hepatology · 2025Review
- Engineering wetware and software for the predictive design of compressed genetic circuits for higher-state decision-making.Nature communications · 2025Article
- Quantum-inspired logic for advanced Transcriptional Programming.Nucleic acids research · 2025Article
- Toward predictable universal genetic circuit design.Quantitative biology (Beijing, China) · 2024Article
- Engineering intelligent chassis cells via recombinase-based MEMORY circuits.Nature communications · 2024Article
- Accelerating Genetic Sensor Development, Scale-up, and Deployment Using Synthetic Biology.Biodesign research · 2024Article
- Next generation synthetic memory via intercepting recombinase function.Nature communications · 2023Article
- Genetic Engineering of Resident Bacteria in the Gut Microbiome.Journal of bacteriology · 2023Review
- Synthesizing cellular LOGIC.Nature chemical biology · 2023Article
- Performance Prediction of Fundamental Transcriptional Programs.ACS synthetic biology · 2023Article
- Systems and synthetic biology-driven engineering of live bacterial therapeutics.Frontiers in bioengineering and biotechnology · 2023Review
- Talking to intractable microbes.Communications biology · 2022Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacteroides species are prominent members of the human gut microbiota. The prevalence and stability of Bacteroides in humans make them ideal candidates to engineer as programmable living therapeutics. Here we report a biotic decision-making technology in a community of Bacteroides (consortium transcriptional programming) with genetic circuit compression. Circuit compression requires systematic pairing of engineered transcription factors with cognate regulatable promoters. In turn, we demonstrate the compression workflow by designing, building, and testing all fundamental two-input logic gates dependent on the inputs isopropyl-β-D-1-thiogalactopyranoside and D-ribose. We then deploy complete sets of logical operations in five human donor Bacteroides, with which we demonstrate sequential gain-of-function control in co-culture. Finally, we couple transcriptional programs with CRISPR interference to achieve loss-of-function regulation of endogenous genes-demonstrating complex control over community composition in co-culture. This work provides a powerful toolkit to program gene expression in Bacteroides for the development of bespoke therapeutic bacteria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.