Evidence map›Paper›PMID 35793554›Full record

ArticleACS synthetic biology2022

High-Throughput Aminoacyl-tRNA Synthetase Engineering for Genetic Code Expansion in Yeast.

Jessica T Stieglitz, James A Van Deventer

Open access · greenAbstract read
In one paragraph

Article in ACS synthetic biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
3.6field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 42 citations in OpenAlex.

  1. Review
  2. Pharmacophore-driven antibody discovery on the yeast surface.bioRxiv : the preprint server for biology · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Jessica T StieglitzChemical and Biological Engineering Department, Tufts University, Medford, Massachusetts 02155, United States.ORCID 0000-0003-3845-0712
James A Van DeventerChemical and Biological Engineering Department, Tufts University, Medford, Massachusetts 02155, United States.ORCID 0000-0003-4343-6157
Tufts University · US

Funding

The yeast surface as a platform for inhibitor discoveryR35GM133471 · NIGMS · TUFTS UNIVERSITY MEDFORD · PI James Allen Van Deventer · 2019 to 2026
$3.1M
BD Influx 5 Laser Fluorescence Activated Cell SorterS10OD016196 · OD · TUFTS UNIVERSITY BOSTON · PI THORLEY-LAWSON, DAVID A. · 2013 to 2013
$600k
NIGMS NIH HHS R35 GM133471NIH HHS S10 OD016196
6 · The paper itself

Abstract

Protein expression with genetically encoded noncanonical amino acids (ncAAs) benefits a broad range of applications, from the discovery of biological therapeutics to fundamental biological studies. A major factor limiting the use of ncAAs is the lack of orthogonal translation systems (OTSs) that support efficient genetic code expansion at repurposed stop codons. Aminoacyl-tRNA synthetases (aaRSs) have been extensively evolved in

Indexed as

Amino Acyl-tRNA SynthetasesAmino AcidsCodon, TerminatorEscherichia coliGenetic CodeProtein EngineeringRNA, TransferSaccharomyces cerevisiaeAmino AcidsAmino Acyl-tRNA SynthetasesCodon, TerminatorRNA, Transferamber suppressionaminoacyl-tRNA synthetasefluorescence-activated cell sortinggenetic code manipulationnoncanonical amino acidspolyspecificityyeast display

Identifiers

PMID35793554
PMCPMC10065163
OpenAlexW4284894834

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.